[The detection of corticoliberin in the neurosecretory cells of additional Gomori-positive centers in the rat hypothalamus].

The experiments were carried out in male Wistar rats in which deafferentation of the mediobasal hypothalamus was performed or colchicine (75 micrograms/10 microliters of saline) was inoculated into the III ventricle cavity. Using the PAP-method, the presence of corticoloberin was determined immunohistochemically in neurosecretory cells of additional groups (AG) of the hypothalamus: circular, perifornical (PFG), dorsolateral, ventrolateral, as well as extrahypothalamically located group (the internal capsule area). The intact rats were shown to have no corticoliberin-immunoreactive (CL-IR) cells in any of AG. After experimental treatments, CL-IR cells appeared in PFG only but not in any other AG. In the experimental rats, two types of CL-IR cells were demonstrated in PFG. The first type consisted of cells of 15-20 microns in diameter with processes, similar to those found in the paraventricular nucleus. The second type consisted of large cells (40-50 microns in diameter) with morphological features of nonapeptidergic ones whose cytoplasm gave a weak reaction with antiserum to corticoliberin. Thus, among AG of various localizations PFG alone contained CL-IR cells which indicated the involvement of this group into realization of the stress reaction.