Grinevich V V, Danilova O A, Chernigovskaia E V, Polenov A L
Biull Eksp Biol Med. 1993 Jul;116(7):13-5.
The experiments were carried out in male Wistar rats in which deafferentation of the mediobasal hypothalamus was performed or colchicine (75 micrograms/10 microliters of saline) was inoculated into the III ventricle cavity. Using the PAP-method, the presence of corticoloberin was determined immunohistochemically in neurosecretory cells of additional groups (AG) of the hypothalamus: circular, perifornical (PFG), dorsolateral, ventrolateral, as well as extrahypothalamically located group (the internal capsule area). The intact rats were shown to have no corticoliberin-immunoreactive (CL-IR) cells in any of AG. After experimental treatments, CL-IR cells appeared in PFG only but not in any other AG. In the experimental rats, two types of CL-IR cells were demonstrated in PFG. The first type consisted of cells of 15-20 microns in diameter with processes, similar to those found in the paraventricular nucleus. The second type consisted of large cells (40-50 microns in diameter) with morphological features of nonapeptidergic ones whose cytoplasm gave a weak reaction with antiserum to corticoliberin. Thus, among AG of various localizations PFG alone contained CL-IR cells which indicated the involvement of this group into realization of the stress reaction.
实验在雄性Wistar大鼠身上进行，对其进行了下丘脑内侧基底部的去传入神经手术，或将秋水仙碱（75微克/10微升生理盐水）注入第三脑室腔。采用过氧化物酶抗过氧化物酶法（PAP法），通过免疫组织化学方法在下丘脑额外组（AG）的神经分泌细胞中检测促肾上腺皮质激素释放素的存在，这些额外组包括环状组、穹窿周组（PFG）、背外侧组、腹外侧组，以及位于下丘脑外的组（内囊区）。结果显示，在任何一个额外组中，正常大鼠均未发现促肾上腺皮质激素释放素免疫反应性（CL-IR）细胞。实验处理后，仅在穹窿周组出现了CL-IR细胞，其他额外组均未出现。在实验大鼠的穹窿周组中，发现了两种类型的CL-IR细胞。第一种类型由直径为15 - 20微米的细胞组成，具有突起，类似于室旁核中的细胞。第二种类型由大细胞（直径40 - 50微米）组成，具有神经肽能细胞的形态特征，其细胞质与抗促肾上腺皮质激素释放素抗血清的反应较弱。因此，在不同定位的额外组中，只有穹窿周组含有CL-IR细胞，这表明该组参与了应激反应的实现。
