Koch A E, Turkiewicz W, Harlow L A, Pope R M
Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611.
Clin Immunol Immunopathol. 1993 Oct;69(1):29-35. doi: 10.1006/clin.1993.1146.
Cellular adhesion molecules, such as E-selectin, function to recruit leukocytes into inflammatory lesions. Recently, a soluble form of this adhesion molecule, sE-selectin, has been described. In this study we determined soluble E-selectin (sE-selectin) in synovial fluid (SF) and blood samples from patients with rheumatoid arthritis (RA) and other inflammatory disorders and correlated sE-selectin levels with clinical parameters of disease activity. SF, blood, and cells isolated from RA SFs and synovial tissues (STs) were examined from 76 patients. In addition, normal plasma as well as supernatants from cultured human umbilical vein endothelial cells (HUVECs) were obtained. sE-selectin levels were assayed in these fluids and cell supernatants by an enzyme-linked immunoabsorbant (ELISA) assay. SFs from patients with RA had significantly higher sE-selectin levels than did those from osteoarthritis (OA) SFs (P < 0.05). SF sE-selectin levels were correlated with SF leukocyte counts. HUVECs, or RA ST cells enriched in endothelial cells, produced sE-selectin. Neutrophils isolated from RA SFs did not release sE-selectin. SF soluble intercellular adhesion molecule-1 levels correlated positively with sE-selectin levels. We conclude that sE-selectin levels are increased in SFs from RA compared to those from OA. Endothelial cells derived from umbilical vein or from RA STs release sE-selectin. Thus, sE-selectin may be important in the migration of inflammatory leukocytes into diseased RA STs and SFs.
细胞黏附分子，如E-选择素，其作用是将白细胞募集到炎症损伤部位。最近，已经描述了这种黏附分子的一种可溶性形式，即可溶性E-选择素（sE-选择素）。在本研究中，我们测定了类风湿关节炎（RA）和其他炎症性疾病患者的滑液（SF）和血液样本中的可溶性E-选择素（sE-选择素），并将sE-选择素水平与疾病活动的临床参数相关联。对76例患者的RA滑液、血液以及从RA滑液和滑膜组织（ST）中分离出的细胞进行了检测。此外，还获得了正常血浆以及培养的人脐静脉内皮细胞（HUVEC）的上清液。通过酶联免疫吸附测定（ELISA）法测定这些液体和细胞上清液中的sE-选择素水平。RA患者的滑液中sE-选择素水平显著高于骨关节炎（OA）患者的滑液（P < 0.05）。滑液sE-选择素水平与滑液白细胞计数相关。富含内皮细胞的HUVEC或RA ST细胞产生sE-选择素。从RA滑液中分离出的中性粒细胞不释放sE-选择素。滑液可溶性细胞间黏附分子-1水平与sE-选择素水平呈正相关。我们得出结论，与OA患者相比，RA患者滑液中的sE-选择素水平升高。源自脐静脉或RA ST的内皮细胞释放sE-选择素。因此，sE-选择素可能在炎症白细胞向患病的RA ST和滑液的迁移中起重要作用。
