Koch A E, Shah M R, Harlow L A, Lovis R M, Pope R M
Department of Medicine, Northwestern University Medical School, Chicago, Illinois 60611.
Clin Immunol Immunopathol. 1994 May;71(2):208-15. doi: 10.1006/clin.1994.1074.
The objective of this study is to determine whether soluble intercellular adhesion molecule-1 (sICAM-1) is found in rheumatoid arthritis (RA) and other inflammatory disorders and to identify which cells are responsible for sICAM-1 production. Synovial fluid, blood and cells isolated from RA synovial fluids, and synovial tissues from 59 patients were studied. In addition, normal peripheral blood was obtained. sICAM-1 was assayed by an enzyme-linked immunoabsorbent assay. Synovial fluids from patients with RA and other inflammatory arthritides had significantly higher sICAM-1 levels than did osteoarthritis (OA) synovial fluids. Synovial fluid sICAM-1 levels were significantly positively correlated with synovial fluid leukocyte counts. RA synovial tissue fibroblasts released low levels of sICAM-1. Neutrophils (PMNs) isolated from synovial fluids of RA patients spontaneously released sICAM-1. However, mononuclear cells isolated from RA synovial fluid produced the largest quantities of sICAM-1. Phytohemagglutinin but not lipopolysaccharide enhanced mononuclear sICAM-1 release. sICAM-1 was increased in synovial fluids from RA compared to OA. This sICAM-1 may be important in modulating the trafficking of inflammatory leukocytes into diseased RA synovial tissue and fluid.
本研究的目的是确定可溶性细胞间黏附分子-1（sICAM-1）是否存在于类风湿关节炎（RA）及其他炎症性疾病中，并确定哪些细胞负责产生sICAM-1。对59例患者的滑液、血液、从RA滑液中分离出的细胞以及滑膜组织进行了研究。此外，还获取了正常外周血。通过酶联免疫吸附测定法检测sICAM-1。RA患者和其他炎性关节炎患者的滑液中sICAM-1水平显著高于骨关节炎（OA）患者的滑液。滑液中sICAM-1水平与滑液白细胞计数显著正相关。RA滑膜组织成纤维细胞释放的sICAM-1水平较低。从RA患者滑液中分离出的中性粒细胞（PMN）可自发释放sICAM-1。然而，从RA滑液中分离出的单核细胞产生的sICAM-1量最大。植物血凝素而非脂多糖可增强单核细胞sICAM-1的释放。与OA相比，RA滑液中的sICAM-1增加。这种sICAM-1可能在调节炎性白细胞向患病RA滑膜组织和滑液的迁移中起重要作用。
