Morikawa K, Miyawaki T, Oseko F, Morikawa S, Imai K
Department of Internal Medicine, Shimane Medical University, Japan.
Eur J Haematol. 1993 Sep;51(3):144-51. doi: 10.1111/j.1600-0609.1993.tb00615.x.
The effect of granulocyte-colony stimulating factor (G-CSF) on human B-cell function was studied in in vitro cultures. G-CSF alone had no effect on the proliferative response of resting B cells, but it slightly enhanced the proliferative response of these cells in the presence of polyclonal B-cell mitogen, Staphylococcus aureus Cowan strain I (SAC) at concentrations of 0.2 to 25 micrograms/ml (1.5-fold increase in the DNA synthesis). In contrast, immunoglobulin (Ig) secretion of activated B cells was increased approximately three-fold to four-fold by adding G-CSF to the cultures. The neutralization of G-CSF bioactivity with anti-G-CSF antibody abrogated this effect. Though cytoplasmic Ig-positive cells or plasma cell marker-positive cells did not change, the expression of IgM mRNA in antibody-producing B cells increased in the presence of G-CSF in the cultures. Interestingly, human B lymphocytes are shown to express the binding to biotin-conjugated G-CSF preparation, but not to biotin-conjugated GM-CSF preparation when examined by flow cytometry. These data suggest that G-CSF may influence B-cell function in special circumstances.
在体外培养中研究了粒细胞集落刺激因子(G-CSF)对人B细胞功能的影响。单独的G-CSF对静息B细胞的增殖反应没有影响,但在浓度为0.2至25微克/毫升的多克隆B细胞有丝分裂原金黄色葡萄球菌考恩I株(SAC)存在下,它略微增强了这些细胞的增殖反应(DNA合成增加1.5倍)。相反,通过向培养物中添加G-CSF,活化B细胞的免疫球蛋白(Ig)分泌增加了约三到四倍。用抗G-CSF抗体中和G-CSF生物活性消除了这种作用。虽然细胞质Ig阳性细胞或浆细胞标志物阳性细胞没有变化,但在培养物中存在G-CSF的情况下,产生抗体的B细胞中IgM mRNA的表达增加。有趣的是,当通过流式细胞术检测时,人B淋巴细胞显示出与生物素偶联的G-CSF制剂结合,但不与生物素偶联的GM-CSF制剂结合。这些数据表明,G-CSF可能在特殊情况下影响B细胞功能。