Malygin A M, Timonen T
Department of Pathology, University of Helsinki, Finland.
Immunology. 1993 Jul;79(3):506-8.
Interleukin-2 (IL-2)-induced generation of non-major histocompatibility complex (MHC)-restricted killer cells among human cord blood lymphocytes (CBL) was investigated. After 1 week in culture with recombinant (r)IL-2 and human serum (HuSer), the cytotoxicity of CBL against K562 and COLO cells greatly exceeded the cytotoxicity of cultured adult peripheral blood lymphocytes. Culturing of CBL with rIL-2 and HuSer led to preferable generation of CD56+ cells. After 1 month in culture, the number and frequency of CD56+ cells had increased by more than 50 and nine times, respectively. The generation of CD56+ cells in CBL cultures may at least partially be explained by their comparatively strong expression of the IL-2 receptor (IL-2R) beta-chain (p75).
研究了白细胞介素-2(IL-2)诱导人脐血淋巴细胞(CBL)产生非主要组织相容性复合体(MHC)限制性杀伤细胞的情况。在用重组(r)IL-2和人血清(HuSer)培养1周后,CBL对K562和COLO细胞的细胞毒性大大超过培养的成人外周血淋巴细胞的细胞毒性。用rIL-2和HuSer培养CBL可导致CD56+细胞的优先产生。培养1个月后,CD56+细胞的数量和频率分别增加了50倍和9倍以上。CBL培养物中CD56+细胞的产生至少部分可以通过它们相对较强地表达IL-2受体(IL-2R)β链(p75)来解释。