Nagler A, Lanier L L, Phillips J H
Becton Dickinson Monoclonal Center, Inc., San Jose, California 95131.
J Exp Med. 1990 May 1;171(5):1527-33. doi: 10.1084/jem.171.5.1527.
The majority of human NK cells express low affinity IgG Fc receptors (CD16+), whereas a minor subset of NK cells lack Fc receptor expression (CD16-). In contrast to CD16+ NK cells that express only p75 IL-2 receptors, CD16- NK cells constitutively co-express both p75 and p55 IL-2 receptors in vivo and preferentially respond to low concentrations of IL-2 with increased cytolytic activation and proliferation. Scatchard analysis demonstrated the presence of approximately 1,200 high affinity (approximately 25 pM kD) and approximately 9,600 intermediate affinity (approximately 2 nM kD) IL-2 receptors on CD16- NK cells. CD16+ NK cells expressed only a single intermediate affinity IL-2 receptor of approximately 1.9 nM kD (approximately 9,000 sites per cell). The IL-2 binding data thus substantiated the phenotypic and functional studies and definitively show that the differential responsiveness of CD16- and CD16+ NK cells to IL-2 is manifested through different affinity IL-2 receptors.
大多数人类自然杀伤细胞（NK细胞）表达低亲和力IgG Fc受体（CD16+），而一小部分NK细胞缺乏Fc受体表达（CD16-）。与仅表达p75白细胞介素2（IL-2）受体的CD16+ NK细胞不同，CD16- NK细胞在体内组成性共表达p75和p55 IL-2受体，并优先对低浓度IL-2作出反应，细胞溶解活性和增殖增加。Scatchard分析表明，CD16- NK细胞上存在约1200个高亲和力（约25 pM kD）和约9600个中等亲和力（约2 nM kD）的IL-2受体。CD16+ NK细胞仅表达单个中等亲和力的IL-2受体，约为1.9 nM kD（每个细胞约9000个位点）。因此，IL-2结合数据证实了表型和功能研究，并明确表明CD16-和CD16+ NK细胞对IL-2的不同反应性是通过不同亲和力的IL-2受体表现出来的。
