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Characterization of an immunoreactive 17.5-kilodalton outer membrane protein of Haemophilus somnus by using a monoclonal antibody.

作者信息

Tagawa Y, Haritani M, Yuasa N

机构信息

National Institute of Animal Health, Ibaraki, Japan.

出版信息

Infect Immun. 1993 Oct;61(10):4153-7. doi: 10.1128/iai.61.10.4153-4157.1993.

DOI:10.1128/iai.61.10.4153-4157.1993
PMID:7691744
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC281138/
Abstract

A single outer membrane protein (OMP) of Haemophilus somnus, with an apparent molecular mass of 17.5 kDa, was identified in the sodium dodecyl sulfate (SDS)-insoluble fraction after extraction with 1% SDS-0.5 M NaCl-0.1% beta-mercaptoethanol. A hybridoma derived from mice immunized with H. somnus OMP fractions produced a monoclonal antibody (MAb), designated 20-3-5, that bound to the 17.5-kDa OMP of H. somnus. The MAb 20-3-5 epitope was present on 45 of 45 strains of H. somnus tested. MAb 20-3-5 cross-reacted with Haemophilus agni, Histophilus ovis, and Haemophilus haemoglobinophilus but not with 13 other species and subspecies of gram-negative bacteria. Immunoelectron-microscopic and antibody absorption studies revealed that the MAb 20-3-5 epitope is exposed on the surface of bacteria. In an immunoblot analysis, convalescent-phase sera obtained from calves with experimental H. somnus pneumonia contained antibodies to the 17.5-kDa OMP of H. somnus. Future studies will be directed toward examining the role of the 17.5-kDa OMP in immunity to H. somnus infections.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f7/281138/3740a81e9705/iai00022-0143-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f7/281138/b40dc19b29e6/iai00022-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f7/281138/916629323c95/iai00022-0142-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f7/281138/6e0f31148438/iai00022-0143-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f7/281138/59c1314de936/iai00022-0143-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f7/281138/0ff1597cf022/iai00022-0143-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f7/281138/3740a81e9705/iai00022-0143-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f7/281138/b40dc19b29e6/iai00022-0142-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f7/281138/916629323c95/iai00022-0142-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f7/281138/6e0f31148438/iai00022-0143-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f7/281138/59c1314de936/iai00022-0143-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f7/281138/0ff1597cf022/iai00022-0143-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/36f7/281138/3740a81e9705/iai00022-0143-d.jpg

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本文引用的文献

1
Antigenic analysis of the major outer membrane protein of Haemophilus somnus with monoclonal antibodies.
Infect Immun. 1993 May;61(5):2257-9. doi: 10.1128/iai.61.5.2257-2259.1993.
2
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Infect Immun. 1993 May;61(5):1750-5. doi: 10.1128/iai.61.5.1750-1755.1993.
3
Purification and partial characterization of the major outer membrane protein of Haemophilus somnus.睡眠嗜血杆菌主要外膜蛋白的纯化及部分特性分析
杜克雷嗜血杆菌保守的18000分子量外膜蛋白与PAL具有同源性。
Infect Immun. 1996 Jun;64(6):1950-5. doi: 10.1128/iai.64.6.1950-1955.1996.
Infect Immun. 1993 Jan;61(1):91-6. doi: 10.1128/iai.61.1.91-96.1993.
4
Cross-reactivity of Haemophilus somnus antibody in agglutination and complement fixation tests and in the enzyme-linked immunosorbent assay.睡眠嗜血杆菌抗体在凝集试验、补体结合试验及酶联免疫吸附测定中的交叉反应性。
J Clin Microbiol. 1983 Mar;17(3):500-6. doi: 10.1128/jcm.17.3.500-506.1983.
5
Morphological, biochemical, antigenic, and cytochemical relationships among Haemophilus somnus, Haemophilus agni, Haemophilus haemoglobinophilus, Histophilus ovis, and Actinobacillus seminis.睡眠嗜血杆菌、羊嗜血杆菌、嗜血红蛋白嗜血杆菌、绵羊组织杆菌和精液放线杆菌之间的形态学、生物化学、抗原性及细胞化学关系。
J Clin Microbiol. 1983 May;17(5):728-37. doi: 10.1128/jcm.17.5.728-737.1983.
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Vaginitis in a bitch associated with Haemophilus sp.一只母犬发生与嗜血杆菌属相关的阴道炎
Am J Vet Res. 1971 Dec;32(12):2067-9.
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Measurement of protein using bicinchoninic acid.使用二辛可宁酸测定蛋白质。
Anal Biochem. 1985 Oct;150(1):76-85. doi: 10.1016/0003-2697(85)90442-7.
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Antigenic characterization of the P6 protein of nontypable Haemophilus influenzae.不可分型流感嗜血杆菌P6蛋白的抗原特性分析
Infect Immun. 1986 Dec;54(3):774-9. doi: 10.1128/iai.54.3.774-779.1986.
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Infect Immun. 1988 Sep;56(9):2307-16. doi: 10.1128/iai.56.9.2307-2316.1988.
10
Characterization of two Haemophilus somnus Fc receptors.
J Gen Microbiol. 1988 Jul;134(7):1993-9. doi: 10.1099/00221287-134-7-1993.