Müller S, Wang H, Silverman G J, Bramlet G, Haigwood N, Köhler H
L.P. Markey Cancer Center, University of Kentucky, Lexington 40536.
Scand J Immunol. 1993 Oct;38(4):327-34. doi: 10.1111/j.1365-3083.1993.tb01734.x.
Antibodies in sera of HIV-1 infected individuals against the HIV-1 core protein (p24), HIV-1 envelope glycoprotein (gp120) and reverse transcriptase (RT) are characterized by a skewed light chain isotype expression. The kappa/lambda ratios of antibodies to p24 and gp120 in infected individuals were found to be unique in each individual, but constant over several years independently from disease progression. The oligoclonal nature of the anti-HIV-1 antibodies suggested by skewed kappa/lambda expression was confirmed with isoelectric focusing of affinity purified antibodies to p24 and gp120. Analysis of the utilization of V gene families in purified anti-p24 and anti-gp120 antibodies revealed a restricted and biased VH gene family usage. In contrast, the utilization of VK gene families appeared to be random. The finding of stable and restricted antibody responses in infected individuals could be one of the causes for the failure to produce antibodies to HIV-1 that are effective against escape virus variants.
人类免疫缺陷病毒1型（HIV-1）感染者血清中针对HIV-1核心蛋白（p24）、HIV-1包膜糖蛋白（gp120）和逆转录酶（RT）的抗体具有轻链同种型表达偏倚的特征。在感染者中，针对p24和gp120抗体的κ/λ比值在每个个体中都是独特的，但在数年中保持恒定，且与疾病进展无关。通过对亲和纯化的针对p24和gp120的抗体进行等电聚焦，证实了由κ/λ表达偏倚所提示的抗HIV-1抗体的寡克隆性质。对纯化的抗p24和抗gp120抗体中V基因家族利用情况的分析显示，VH基因家族的使用受到限制且存在偏倚。相比之下，VK基因家族的使用似乎是随机的。在感染者中发现稳定且受限的抗体反应可能是无法产生有效对抗逃逸病毒变体的HIV-1抗体的原因之一。
