Jensen P, Fomsgaard A, Shand G, Hindersson P, Høiby N
Department of Medical Microbiology, University of Copenhagen, Denmark.
APMIS. 1993 Aug;101(8):621-30. doi: 10.1111/j.1699-0463.1993.tb00156.x.
Quantitative crossed immunoelectrophoresis was used to evaluate the antigenic similarity of Pseudomonas aeruginosa and Pseudomonas cepacia GroEL proteins. We found that the two proteins showed 75% identity. By using a panel of monoclonal antibodies against the P. aeruginosa GroEL protein, we identified 10 monoclonal antibodies which cross-reacted with the P. cepacia GroEL protein and 21 monoclonal antibodies which recognized type-specific epitopes on the P. aeruginosa GroEL protein. In crossed immunoelectrophoresis two different fractions of GroEL reactive material could be resolved. These fractions showed a reaction of partial identity. Examination of the two immunoprecipitates by Western blotting, showed that both fractions consisted of anti-60 kDa GroEL reactive protein. One fraction, in addition, contained LPS with a characteristic 'ladder' reaction in modified Western blotting. We therefore conclude that this fraction represents a complex between LPS and GroEL.
采用定量交叉免疫电泳法评估铜绿假单胞菌和洋葱伯克霍尔德菌GroEL蛋白的抗原相似性。我们发现这两种蛋白的同一性为75%。通过使用一组抗铜绿假单胞菌GroEL蛋白的单克隆抗体，我们鉴定出10种与洋葱伯克霍尔德菌GroEL蛋白发生交叉反应的单克隆抗体以及21种识别铜绿假单胞菌GroEL蛋白上型特异性表位的单克隆抗体。在交叉免疫电泳中，可分辨出GroEL反应性物质的两个不同组分。这些组分显示出部分同一性反应。通过蛋白质印迹法检测这两种免疫沉淀物，结果表明两个组分均由抗60 kDa GroEL反应性蛋白组成。此外，其中一个组分在改良蛋白质印迹法中含有具有特征性“梯状”反应的脂多糖。因此，我们得出结论，该组分代表脂多糖与GroEL之间的复合物。
