Lubrano C, Toscano V, Petrangeli E, Spera G, Trotta M C, Rombolà N, Frati L, Di Silverio F, Sciarra F
Department of Endocrinology III, University La Sapienza, Rome, Italy.
J Steroid Biochem Mol Biol. 1993 Oct;46(4):463-8. doi: 10.1016/0960-0760(93)90100-b.
Human benign prostatic hyperplasia (BPH) samples were analyzed to evaluate the presence of immunoreactive epidermal growth factor (irEGF) and EGF receptor (EGFR). In all BPH samples examined both peptide and its receptor were present. Scatchard analysis of binding data of [125I]EGF showed two classes of binding sites with high and low affinity. Intratissular irEGF concentrations showed a significant inverse linear correlation with EGFR levels. Two groups of samples can be identified: the first showing high irEGF concentrations and low levels of EGF binding sites; the second low irEGF and high concentrations of EGFR. The simultaneous presence of EGF and its receptor in BPH samples indicates that this growth factor may act in an autocrine/paracrine manner in human prostatic tissue. The inverse relationship between EGF and the two sites of EGFR lead one to hypothesize that EGF itself could play a central role in determining receptor cell surface availability.
对人良性前列腺增生（BPH）样本进行分析，以评估免疫反应性表皮生长因子（irEGF）和表皮生长因子受体（EGFR）的存在情况。在所有检测的BPH样本中，该肽及其受体均存在。对[125I]EGF结合数据的Scatchard分析显示有两类具有高亲和力和低亲和力的结合位点。组织内irEGF浓度与EGFR水平呈显著的负线性相关。可识别出两组样本：第一组显示irEGF浓度高且EGF结合位点水平低；第二组显示irEGF低且EGFR浓度高。BPH样本中EGF及其受体的同时存在表明，这种生长因子可能在人前列腺组织中以自分泌/旁分泌方式发挥作用。EGF与EGFR的两个位点之间的反向关系使人推测EGF本身可能在决定受体细胞表面可用性方面发挥核心作用。
