固定化核酸酶

Immobilized nucleases.

作者信息

Reddy L G, Shankar V

机构信息

Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.

出版信息

Crit Rev Biotechnol. 1993;13(3):255-73. doi: 10.3109/07388559309041320.

DOI:10.3109/07388559309041320

PMID:7693354

Abstract

Nucleases are important analytical enzymes and are used widely for the determination of nucleic acid structure. Their application depends on the specificity and mode of action of the particular enzyme. Nucleases have also found application in the production of flavor enhancers like 5' IMP and 5' GMP, removal of nucleic acids in single cell protein preparations, and as therapeutic agents. Immobilization of nucleases and the use of immobilized nucleases for various biotechnological applications have been reviewed.

摘要

核酸酶是重要的分析酶，被广泛用于核酸结构的测定。它们的应用取决于特定酶的特异性和作用方式。核酸酶还被用于生产风味增强剂，如5'-肌苷酸二钠（5' IMP）和5'-鸟苷酸二钠（5' GMP），去除单细胞蛋白制剂中的核酸，以及作为治疗剂。本文综述了核酸酶的固定化以及固定化核酸酶在各种生物技术应用中的使用情况。