Doğan A, MacDonald T T, Spencer J
Department of Histopathology, University College London Medical School, GB.
Eur J Immunol. 1993 Nov;23(11):2922-6. doi: 10.1002/eji.1830231129.
In a fetal intestinal organ culture model of intestinal inflammation, activation of T cells by a lectin, superantigen or anti-CD3 antibodies induces E-selectin expression which peaks 6-10 h after activation and disappears by 24 h. We now show that addition of cyclosporin A (Cys A) increases the number of E-selectin-expressing endothelial cells and the intensity of expression, and it prolongs expression up to at least 60 h after stimulation. The dynamics of E-selectin expression on tumor necrosis factor activated human umbilical vein endothelial cell cultures is not affected by Cys A. Furthermore, other immunosuppressive agents FK506 and dexamethasone inhibit E-selectin expression, indicating that the enhancement observed in the presence of Cys A is not a consequence of general immunosuppression.
在肠道炎症的胎儿肠道器官培养模型中，凝集素、超抗原或抗CD3抗体激活T细胞可诱导E-选择素表达，该表达在激活后6至10小时达到峰值，并在24小时时消失。我们现在表明，添加环孢素A（Cys A）可增加表达E-选择素的内皮细胞数量和表达强度，并将表达延长至刺激后至少60小时。Cys A不影响肿瘤坏死因子激活的人脐静脉内皮细胞培养物中E-选择素表达的动力学。此外，其他免疫抑制剂FK506和地塞米松可抑制E-选择素表达，表明在Cys A存在下观察到的增强不是一般免疫抑制的结果。
