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通过聚丙烯酰胺凝胶银染法在空肠弯曲菌不同热稳定血清型中显示带有O-多糖链的脂多糖

Demonstration of lipopolysaccharide with O-polysaccharide chains among different heat-stable serotypes of Campylobacter jejuni by silver staining of polyacrylamide gels.

作者信息

Blake D C, Russell R G

机构信息

Program of Comparative Medicine, School of Medicine, University of Maryland at Baltimore.

出版信息

Infect Immun. 1993 Dec;61(12):5384-7. doi: 10.1128/iai.61.12.5384-5387.1993.

DOI:10.1128/iai.61.12.5384-5387.1993
PMID:7693600
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC281326/
Abstract

Lipopolysaccharide (LPS) from three human and four monkey isolates of Campylobacter jejuni was extracted in high yields that revealed ladder-like structures in polyacrylamide gels by direct silver staining. These observations demonstrate that C. jejuni possesses LPS with O-chain repeating units typical of the family Enterobacteriaceae. The isolates showed differences in the number and electrophoretic mobility of bands in silver-stained gels.

摘要

从三株人源和四株猴源空肠弯曲菌分离株中提取的脂多糖（LPS）产量很高，通过直接银染在聚丙烯酰胺凝胶中呈现出梯状结构。这些观察结果表明，空肠弯曲菌拥有具有肠杆菌科典型O链重复单元的LPS。这些分离株在银染凝胶中的条带数量和电泳迁移率上存在差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf09/281326/9f0889b48113/iai00024-0456-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf09/281326/660609c84689/iai00024-0455-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf09/281326/a28fa2f4897b/iai00024-0455-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf09/281326/9f0889b48113/iai00024-0456-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf09/281326/660609c84689/iai00024-0455-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf09/281326/a28fa2f4897b/iai00024-0455-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cf09/281326/9f0889b48113/iai00024-0456-a.jpg

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本文引用的文献

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Isolation and chemical composition of lipopolysaccharide from Campylobacter jejuni.空肠弯曲菌脂多糖的分离及化学成分分析
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