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基于可溶性热稳定抗原的胎儿弯曲菌空肠亚种血清分型的被动血凝技术。

Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens.

作者信息

Penner J L, Hennessy J N

出版信息

J Clin Microbiol. 1980 Dec;12(6):732-7. doi: 10.1128/jcm.12.6.732-737.1980.

Abstract

Antigenic materials were extracted from Campylobacter fetus subsp. jejuni strains by heating bacterial suspensions in saline at 100 degrees C and by exposure to ethylenediaminetetraacetic acid. The antigens were heat stable at 100 degrees C, capable of sensitizing sheep erythrocytes for agglutination in antisera, and able to elicit production of specific antibody in rabbits; they occurred with different immunological specificities in 23 strains. Antisera against the 23 strains could be used for discriminating among isolates of the species when the passive hemagglutination technique was used for serotyping. Three serotypes were more common than others among a collection of human isolates.

摘要

通过在盐水中于100℃加热细菌悬液以及使其暴露于乙二胺四乙酸,从空肠弯曲菌胎儿亚种菌株中提取抗原性物质。这些抗原在100℃时热稳定,能够使绵羊红细胞致敏以便在抗血清中发生凝集,并且能够在兔体内引发特异性抗体的产生;它们在23个菌株中呈现出不同的免疫特异性。当采用被动血凝技术进行血清分型时,针对这23个菌株的抗血清可用于区分该菌种的分离株。在一组人类分离株中,三种血清型比其他血清型更为常见。

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