Mills S D, Bradbury W C, Penner J L
Infect Immun. 1985 Oct;50(1):284-91. doi: 10.1128/iai.50.1.284-291.1985.
Lipopolysaccharides (LPS) were extracted from eight strains of Campylobacter jejuni and purified by enzyme treatment to remove traces of RNA, DNA, and protein. This material was used to sensitize sheep erythrocytes for the passive hemagglutination assay that is presently used to serotype C. jejuni. The results confirmed that the thermostable antigen typing scheme is based on LPS (O) antigens. The LPS after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining was found to consist of a series of slow migrating bands which could not be eliminated by treatment with NaOH, urea, or EDTA. However, the use of LPS double labeled with 14C and 32P yielded evidence that the bands of high molecular weight were indeed aggregations of low-molecular-weight LPS molecules.
从八株空肠弯曲杆菌中提取脂多糖（LPS），并通过酶处理进行纯化，以去除痕量的RNA、DNA和蛋白质。该材料用于使绵羊红细胞致敏，用于目前用于空肠弯曲杆菌血清分型的被动血凝试验。结果证实，热稳定抗原分型方案基于LPS（O）抗原。经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和银染后的LPS被发现由一系列迁移缓慢的条带组成，用氢氧化钠、尿素或乙二胺四乙酸处理无法消除这些条带。然而，使用用14C和32P双重标记的LPS提供的证据表明，高分子量条带确实是低分子量LPS分子的聚集物。
