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改良混合细胞凝集反应(根据大卫索恩的MCAR)在法医血清学中的应用;胶带MCAR(作者译)

[Application of the modified mixed cell agglutination reaction (MCAR according to Davidsohn) in forensic serology; MCAR on adhesive tape (author's transl)].

作者信息

Ishiyama I, Okada T

出版信息

Z Rechtsmed. 1975 Dec 30;77(1):25-40. doi: 10.1007/BF02114607.

Abstract

In view of serological sensitivity, specificity and simple procedure of reaction pattern the modified mixed cell agglutination reaction (Davidsohn's MCAR) proved to be a very useful test for typing ABO isoantigens in forensic medicine. Its sensitivity surpasses in some cases, where very small amounts of isoantigens have to be detected, that of the absorption-elution reaction several thousand times. The capacity to demonstrate the localization of antigens seems to be more reliable than the original test method. The modified Davidsohn's MCAR (MCAR on adhesive tape) is expecially recommended when isoantigens which are localized on the surface of microscopic objects including fingerprints, dust, hairs, and histological sections, have to be determined. The usefulness of our modification in forensic medicine and the conditions which are essential for the application, are discussed.

摘要

鉴于血清学的敏感性、特异性以及反应模式的操作简便性,改良的混合细胞凝集反应(戴维森氏MCAR)被证明是法医学中ABO同种抗原分型的一种非常有用的检测方法。在某些必须检测极少量同种抗原的情况下,其敏感性比吸收 - 洗脱反应高出数千倍。显示抗原定位的能力似乎比原始检测方法更可靠。当必须确定存在于包括指纹、灰尘、毛发和组织切片等微观物体表面的同种抗原时,特别推荐使用改良的戴维森氏MCAR(胶带法MCAR)。本文讨论了我们的改良方法在法医学中的实用性以及应用所必需的条件。

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