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改良混合细胞凝集反应(根据大卫索恩的MCAR)在法医血清学中的应用;胶带MCAR(作者译)

[Application of the modified mixed cell agglutination reaction (MCAR according to Davidsohn) in forensic serology; MCAR on adhesive tape (author's transl)].

作者信息

Ishiyama I, Okada T

出版信息

Z Rechtsmed. 1975 Dec 30;77(1):25-40. doi: 10.1007/BF02114607.

DOI:10.1007/BF02114607
PMID:769391
Abstract

In view of serological sensitivity, specificity and simple procedure of reaction pattern the modified mixed cell agglutination reaction (Davidsohn's MCAR) proved to be a very useful test for typing ABO isoantigens in forensic medicine. Its sensitivity surpasses in some cases, where very small amounts of isoantigens have to be detected, that of the absorption-elution reaction several thousand times. The capacity to demonstrate the localization of antigens seems to be more reliable than the original test method. The modified Davidsohn's MCAR (MCAR on adhesive tape) is expecially recommended when isoantigens which are localized on the surface of microscopic objects including fingerprints, dust, hairs, and histological sections, have to be determined. The usefulness of our modification in forensic medicine and the conditions which are essential for the application, are discussed.

摘要

鉴于血清学的敏感性、特异性以及反应模式的操作简便性,改良的混合细胞凝集反应(戴维森氏MCAR)被证明是法医学中ABO同种抗原分型的一种非常有用的检测方法。在某些必须检测极少量同种抗原的情况下,其敏感性比吸收 - 洗脱反应高出数千倍。显示抗原定位的能力似乎比原始检测方法更可靠。当必须确定存在于包括指纹、灰尘、毛发和组织切片等微观物体表面的同种抗原时,特别推荐使用改良的戴维森氏MCAR(胶带法MCAR)。本文讨论了我们的改良方法在法医学中的实用性以及应用所必需的条件。

相似文献

1
[Application of the modified mixed cell agglutination reaction (MCAR according to Davidsohn) in forensic serology; MCAR on adhesive tape (author's transl)].改良混合细胞凝集反应(根据大卫索恩的MCAR)在法医血清学中的应用;胶带MCAR(作者译)
Z Rechtsmed. 1975 Dec 30;77(1):25-40. doi: 10.1007/BF02114607.
2
The determination of isoantigenic activity from latent fingerprints: mixed cell agglutination reaction in forensic serology.从潜在指纹中测定同种抗原活性:法医血清学中的混合细胞凝集反应。
J Forensic Sci. 1977 Apr;22(2):365-75.
3
Immunohistological analysis of intravascular agglutination in a case of ABO incompatible blood transfusion by means of mixed cell agglutination reaction.通过混合细胞凝集反应对一例ABO血型不相容输血病例中的血管内凝集进行免疫组织学分析。
Acta Pathol Jpn. 1977 Sep;27(5):729-38. doi: 10.1111/j.1440-1827.1977.tb00188.x.
4
[Single-stage detection of A and B antigens in the hair by absorption-elution and mixed agglutination techniques].[通过吸收-洗脱和混合凝集技术对头发中A和B抗原进行单阶段检测]
Sud Med Ekspert. 2009 Jul-Aug;52(4):24-5.
5
[Use of an anti-H extract of elder berries in the absorption-elution reaction during the examination of blood stains, saliva and sperm].
Sud Med Ekspert. 1989 Jan-Mar;32(1):32-5.
6
[Detection of ABO antigens in stained histological sections by absorption-elution and mixed agglutination reactions. A preliminary report].[通过吸收-洗脱和混合凝集反应在染色组织切片中检测ABO抗原。初步报告]
Sud Med Ekspert. 1974 Jul-Sep;17(3):17-9.
7
["Mixed agglutination" in the study of some objects of forensic medical expertise interest].法医鉴定某些对象研究中的“混合凝集反应”
Sud Med Ekspert. 1973 Jan-Mar;16(1):22-6.
8
[Determination of the antigens of the ABO system in histological sections by the reactions of mixed agglutination and agglutinin absorption (Report I)].
Sud Med Ekspert. 1975 Jul-Sep;18(3):28-9.
9
[Absorption method and mixed agglutination. Comparative studies on the secretor status (author's transl)].[吸收法与混合凝集反应。分泌状态的比较研究(作者译)]
Z Rechtsmed. 1974 May 20;74(2):87-98. doi: 10.1007/BF02114695.
10
[Blood group determination of a single hair].
Beitr Gerichtl Med. 1992;50:139-43.

引用本文的文献

1
[Immunoenzyme determination of ABO and secretor status in paraffin-embedded autopsy material].[石蜡包埋尸检材料中ABO血型及分泌状态的免疫酶测定]
Z Rechtsmed. 1984;93(4):289-300. doi: 10.1007/BF00198655.
2
Immunocytochemical typing of ABO blood groups in vaginal swabs partly contaminated with semen.
Int J Legal Med. 1991 Mar;104(2):87-91. doi: 10.1007/BF01626037.
3
Problems in determing A2B group specific properties in blood stains.确定血迹中A2B血型特异性特征的问题。
Z Rechtsmed. 1977 May 27;79(4):285-8. doi: 10.1007/BF00201170.

本文引用的文献

1
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MIXED AGGLUTINATION WITH TISSUE SECTIONS.组织切片的混合凝集反应。
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IDENTIFICATION OF HUMAN BLOODSTAINS BY MEANS OF THE MIXED ANTIGLOBULIN REACTION ON SEPARATE CLOTH FIBRILS.通过在单独的布纤维上进行混合抗球蛋白反应鉴定人血痕
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[Supplemental remarks on the work by Maresch and Wehrschütz on the secretor character].[关于马雷施和韦尔舒茨所著关于分泌型特征研究的补充说明]
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Early immunologic diagnosis and prognosis of carcinoma. Philip Levine Award Address.癌的早期免疫诊断与预后。菲利普·莱文奖获奖致辞。
Am J Clin Pathol. 1972 Jun;57(6):715-30. doi: 10.1093/ajcp/57.6.715.
10
[Absorption method and mixed agglutination. Comparative studies on the secretor status (author's transl)].[吸收法与混合凝集反应。分泌状态的比较研究(作者译)]
Z Rechtsmed. 1974 May 20;74(2):87-98. doi: 10.1007/BF02114695.