Seelbach A, Fissler B, Madle S
Max-von-Pettenkofer-Institute, Federal Health Office, Berlin, Germany.
Mutat Res. 1993 Dec;303(4):163-9. doi: 10.1016/0165-7992(93)90018-q.
In an earlier publication, we reported on the development of a modified micronucleus assay with V79 cells enabling preferential detection of aneugen-induced micronuclei (Seelbach et al., 1993). Here we present a further evaluation of the modified micronucleus assay based on the investigation of seven further suspected aneugens. Five compounds gave positive results: cadmium chloride, chloral hydrate, hydroquinone, thimerosal and vinblastine. Econazole and pyrimethamine were negative. Up to now, our experience has shown that data produced by the modified V79/micronucleus assay are quite reliable: the variation of spontaneous micronucleus frequencies was low (0.8-1.7%) and the reproducibility of the data was good.
在早期的一篇出版物中，我们报道了一种用V79细胞改进的微核试验的开发，该试验能够优先检测非整倍体诱导的微核（Seelbach等人，1993年）。在此，我们基于对另外七种疑似非整倍体剂的研究，对改进的微核试验进行了进一步评估。五种化合物给出了阳性结果：氯化镉、水合氯醛、对苯二酚、硫柳汞和长春碱。益康唑和乙胺嘧啶为阴性。到目前为止，我们的经验表明，改进的V79/微核试验产生的数据相当可靠：自发微核频率的变化很低（0.8 - 1.7%），并且数据的可重复性良好。
