Desprès L, Imbert-Establet D, Monnerot M
URA CNRS 698, Centre de Biologie et d'Ecologie Tropicale et Méditerranéenne, Université de Perpignan, France.
Mol Biochem Parasitol. 1993 Aug;60(2):221-9. doi: 10.1016/0166-6851(93)90133-i.
Mitochondrial DNA was analyzed from 6 strains of Schistosoma mansoni with 12 restriction enzymes, corresponding to 40 restriction sites or about 1.5% of the coding region. An extensive length polymorphism among strains of S. mansoni was found, with size ranging from 16,500 bp to 24,900 bp. Five restriction sites among 40 were polymorphic; phylogenetic analysis using parsimony criteria supports the idea that American strains were introduced very recently from Africa. Two large fragments (2800 and 5400 bp), representing half of the coding region were cloned. The location of some genes was determined using heterospecific multigenes probes and suggests that this platyhelminth genome differs from all other known mtDNA gene organization.
用12种限制性内切酶对6个曼氏血吸虫株的线粒体DNA进行了分析，这些酶对应40个限制性位点，约占编码区的1.5%。发现曼氏血吸虫株之间存在广泛的长度多态性，大小范围从16500碱基对到24900碱基对。40个限制性位点中有5个是多态性的；使用简约标准进行的系统发育分析支持这样的观点，即美洲株是最近从非洲引入的。代表编码区一半的两个大片段（2800和5400碱基对）被克隆。使用异源多基因探针确定了一些基因的位置，这表明这种扁形虫基因组与所有其他已知的线粒体DNA基因组织不同。
