Willis M C, Hicke B J, Uhlenbeck O C, Cech T R, Koch T H
Department of Chemistry and Biochemistry, University of Colorado, Boulder 80309.
Science. 1993 Nov 19;262(5137):1255-7. doi: 10.1126/science.7694369.
5-Iodouracil-substituted RNA and DNA were crosslinked regiospecifically to associated proteins in yields of 70 to 94% of bound nucleic acid. Irradiation of the iodouracil chromophore with monochromatic, long-wavelength ultraviolet radiation (325 nanometers) eliminates excitation of other nucleic acid and protein chromophores. The combination of high crosslinking yields, excellent specificity, and elimination of photodamage to other chromophores represents an important advance toward the precise identification of contacts in nucleoprotein complexes.
5-碘尿嘧啶取代的RNA和DNA与相关蛋白质发生区域特异性交联，产率为结合核酸的70%至94%。用单色长波长紫外线（325纳米）照射碘尿嘧啶发色团可消除其他核酸和蛋白质发色团的激发。高交联产率、出色的特异性以及消除对其他发色团的光损伤相结合，代表了在精确识别核蛋白复合物中相互作用方面的一项重要进展。
