Bovine L-selectin: a peripheral lymphocyte homing receptor.

The cDNA clone encoding for the bovine peripheral lymph node homing receptor (L-selectin) was isolated and sequenced. The predicted amino acid sequence of bovine L-selectin showed an overall high identity with that of human and murine L-selectin. However, the cytoplasmic tail of bovine L-selectin showed little similarity to that of human and murine L-selectin. The monoclonal antibody DREG-56, which recognizes human L-selectin, blocked the binding of bovine peripheral blood lymphocytes to high-walled endothelial venules in murine peripheral lymph nodes. Surface expression of bovine L-selectin was high in lymphocytes isolated from peripheral lymph nodes and low in lymphocytes isolated from Peyer's patches. This evidence strongly suggests that bovine L-selectin is a peripheral lymphocyte homing receptor.