Bosworth B T, Dowbenko D, Shuster D E, Harp J A
United States Department of Agriculture, National Animal Disease Center, Ames, IA 50010-0070.
Vet Immunol Immunopathol. 1993 Aug;37(3-4):201-15. doi: 10.1016/0165-2427(93)90194-9.
The cDNA clone encoding for the bovine peripheral lymph node homing receptor (L-selectin) was isolated and sequenced. The predicted amino acid sequence of bovine L-selectin showed an overall high identity with that of human and murine L-selectin. However, the cytoplasmic tail of bovine L-selectin showed little similarity to that of human and murine L-selectin. The monoclonal antibody DREG-56, which recognizes human L-selectin, blocked the binding of bovine peripheral blood lymphocytes to high-walled endothelial venules in murine peripheral lymph nodes. Surface expression of bovine L-selectin was high in lymphocytes isolated from peripheral lymph nodes and low in lymphocytes isolated from Peyer's patches. This evidence strongly suggests that bovine L-selectin is a peripheral lymphocyte homing receptor.
编码牛外周淋巴结归巢受体（L-选择素）的cDNA克隆被分离并测序。预测的牛L-选择素氨基酸序列与人和鼠L-选择素的序列总体上具有高度同源性。然而，牛L-选择素的胞质尾部与人及鼠L-选择素的胞质尾部几乎没有相似性。识别人类L-选择素的单克隆抗体DREG-56可阻断牛外周血淋巴细胞与鼠外周淋巴结中高壁内皮微静脉的结合。从外周淋巴结分离的淋巴细胞中牛L-选择素的表面表达较高，而从派伊尔结分离的淋巴细胞中其表达较低。这一证据有力地表明牛L-选择素是一种外周淋巴细胞归巢受体。
