Arbonés M L, Ord D C, Ley K, Ratech H, Maynard-Curry C, Otten G, Capon D J, Tedder T F
Cell Genesys, Incorporated, Foster City, California 94404.
Immunity. 1994 Jul;1(4):247-60. doi: 10.1016/1074-7613(94)90076-0.
L-selectin, a cell adhesion molecule expressed by leukocytes, mediates the attachment of lymphocytes to high endothelial venules (HEV) of peripheral lymph nodes and mediates the earliest interactions between leukocytes and activated vascular endothelium. Mice possessing a mutant L-selectin gene that results in the complete loss of cell surface receptor expression were generated by gene targeting. Lymphocytes from these mice did not bind to peripheral lymph node HEV and these mice had a severe reduction in the number of lymphocytes localized to peripheral lymph nodes. Short-term homing experiments demonstrated that L-selectin was also involved in lymphocyte migration to mucosal lymph nodes, Peyer's patches, and spleen. Furthermore, significant defects in leukocyte rolling and neutrophil migration into the peritoneum in response to an inflammatory stimulus were observed. Thus, L-selectin plays an essential role in leukocyte homing to lymphoid tissues and sites of inflammation.
L-选择素是一种由白细胞表达的细胞黏附分子，介导淋巴细胞与外周淋巴结的高内皮微静脉（HEV）附着，并介导白细胞与活化血管内皮之间的最早相互作用。通过基因靶向技术培育出了具有突变L-选择素基因的小鼠，该突变导致细胞表面受体表达完全丧失。这些小鼠的淋巴细胞不能与外周淋巴结HEV结合，并且在外周淋巴结中定位的淋巴细胞数量严重减少。短期归巢实验表明，L-选择素也参与淋巴细胞向黏膜淋巴结、派尔集合淋巴结和脾脏的迁移。此外，观察到在炎症刺激下白细胞滚动和中性粒细胞向腹膜迁移存在明显缺陷。因此，L-选择素在白细胞归巢至淋巴组织和炎症部位中起着至关重要的作用。
