Sviatchenko V A, Pereboev A V, Agapov E V, Razumov I A, Sabirov A N, Mizenko G A, Samukov V V, Loktev V B
Vopr Virusol. 1993 Jul-Aug;38(4):162-7.
Nine peptides were synthesized for detailed mapping of VEE virus E2-2 and E2-6 sites responsible for the formation of protective antibodies that neutralize the virus and block hemagglutination. The sequence of the peptides over-lapped the regions of amino acid residues 30-75 and 202-250 of VEE virus E2 protein in which antigenic mutations caused by monoclonal antibodies to E2-2 and E2-6 sites had been mapped. None of the synthesized peptides reacted in the enzyme immunoassay with a panel of 17 Mabs to VEE virus E2 protein. However, eight peptides reacted with polyclonal antiviral serum and two of them elicited antiviral antibody production. The E2-2 site might be associated with amino acid residues 30-45, and the region of E2 residues 57-62 in which antigenic mutations are observed is not a linear type antigenic determinant, but participates in the formation of antigenic determinants of the conformational type. The mapping of residues 202-250 demonstrated that all the peptides in this region were well recognized by polyclonal antiviral serum. The residues 235-240 were shown to form a linear epitope which provided a crossover between VEE and EEE viruses and was not recognized by 19 types of monoclonal antibodies cross-reacting with VEE and EEE viruses.
合成了9种肽，用于详细绘制委内瑞拉马脑炎病毒（VEE病毒）E2 - 2和E2 - 6位点的图谱，这些位点负责形成中和病毒并阻断血凝的保护性抗体。肽的序列与VEE病毒E2蛋白的氨基酸残基30 - 75和202 - 250区域重叠，在该区域已绘制了针对E2 - 2和E2 - 6位点的单克隆抗体引起的抗原突变。在酶免疫测定中，合成的肽与一组针对VEE病毒E2蛋白的17种单克隆抗体均无反应。然而，8种肽与多克隆抗病毒血清反应，其中2种引发了抗病毒抗体的产生。E2 - 2位点可能与氨基酸残基30 - 45相关，观察到抗原突变的E2残基57 - 62区域不是线性型抗原决定簇，而是参与构象型抗原决定簇的形成。对残基202 - 250的图谱分析表明，该区域的所有肽都能被多克隆抗病毒血清很好地识别。已证明残基235 - 240形成一个线性表位，该表位在VEE病毒和东部马脑炎病毒（EEE病毒）之间提供了一个交叉区域，并且不被19种与VEE病毒和EEE病毒交叉反应的单克隆抗体识别。
