Hunt A R, Roehrig J T
Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, CO 80522, USA.
Vaccine. 1995 Feb;13(3):281-8. doi: 10.1016/0264-410x(95)93315-z.
In order to define more precisely the protective epitope encoded within the first 25 amino acids (aa) of the E2 glycoprotein of the Trinidad donkey strain of Venezuelan equine encephalomyelitis (VEE) virus, we examined the immunogenicity of smaller peptides within the first 19 aa. pep1-9 and pep3-10 elicited virus-reactive antibody, but failed to protect mice from virus challenge. Additionally, pep3-10 was identified by a competitive binding assay using overlapping peptide octamers as the putative binding site of the antipeptide monoclonal antibody (mAb) 1A2B-10. Since the E2 amino-terminal sequence for all VEE subtype viruses is conserved, we tested the protective capacity in mice of passively transferred mAb 1A2B-10 and found it to protect from both epizootic and enzootic VEE virus challenge. Since horses are an important natural host for VEE virus, pep1-19 was used to immunize horses and was found to be immunogenic and to elicit virus-reactive antibody.
为了更精确地界定委内瑞拉马脑炎（VEE）病毒特立尼达驴毒株E2糖蛋白前25个氨基酸（aa）内编码的保护性表位，我们检测了前19个氨基酸内较小肽段的免疫原性。肽段1-9和肽段3-10可诱导出病毒反应性抗体，但未能保护小鼠免受病毒攻击。此外，通过使用重叠八聚体肽作为抗肽单克隆抗体（mAb）1A2B-10的假定结合位点的竞争结合试验，确定了肽段3-10。由于所有VEE亚型病毒的E2氨基末端序列是保守的，我们检测了被动转移的单克隆抗体1A2B-10在小鼠中的保护能力，发现它可保护小鼠免受流行性和地方性VEE病毒攻击。由于马是VEE病毒的重要自然宿主，因此使用肽段1-19免疫马匹，发现其具有免疫原性并可诱导出病毒反应性抗体。
