委内瑞拉马脑炎病毒保护性相关的四个抗原位点的定位
Localization of four antigenic sites involved in Venezuelan equine encephalomyelitis virus protection.
作者信息
Agapov E V, Razumov I A, Frolov I V, Kolykhalov A A, Netesov S V, Loktev V B
机构信息
Institute of Molecular Biology, State Research Center of Virology and Biotechnology Vector, Koltsovo, Russia.
出版信息
Arch Virol. 1994;139(1-2):173-81. doi: 10.1007/BF01309462.
Stable neutralization and protection escape variants of a virulent strain (Trinidad Donkey) of the VEE virus were selected by monoclonal antibodies (MAbs). Determination of nucleotide sequences of nine variants revealed a clustering of single mutations in four regions of the E1 and E2 glycoproteins. Involvement of amino acid residues 206 (site E1-1), 57 and 59 (site E2-2), 180, 182, 213, 214 and 216 (site E2-6) and 232 (site E2-3) in protective epitopes was demonstrated.
通过单克隆抗体（MAb）筛选出委内瑞拉马脑炎病毒（VEE病毒）强毒株（特立尼达驴毒株）的稳定中和及保护性逃逸变异株。对九个变异株的核苷酸序列测定显示，E1和E2糖蛋白四个区域存在单突变聚集现象。证实了206位氨基酸残基（E1-1位点）、57和59位氨基酸残基（E2-2位点）、180、182、213、214和216位氨基酸残基（E2-6位点）以及232位氨基酸残基（E2-3位点）参与保护性表位。
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