Kantrong S, Sako N
Faculty of Agriculture, Saga University, Japan.
Arch Virol. 1993;133(1-2):11-20. doi: 10.1007/BF01309740.
Fourteen monoclonal antibodies (MAbs) were prepared against two strains of turnip mosaic virus (TuMV) differing in aphid transmissibility. Serological specificity of fourteen MAbs against the two strains was tested by indirect ELISA. Three MAbs were able to distinguish aphid transmissible TuMV strain 1 from non-aphid transmissible strain 31 while four MAbs reacted only with strain 31. No cross-reactivity between the two strains was found using these specific MAbs. Based upon the ability of Mab to inhibit the reaction of other MAbs, antibody competition test indicated that fourteen MAbs recognized six different epitopes on the virus particle; MAbs specific to strain 1 recognized two epitopes while MAbs specific to strain 31 also recognized two epitopes. The remaining two epitopes are common. Since the six amino acid differences between the coat proteins of the two strains were found at the N-terminal regions, MAbs specific to strain 1 or 31 bound to the different epitopes on the N-terminal regions in coat proteins of the two strains.
制备了14种针对两种在蚜虫传播性上存在差异的芜菁花叶病毒（TuMV）毒株的单克隆抗体（MAb）。通过间接酶联免疫吸附测定（ELISA）检测了这14种MAb针对这两种毒株的血清学特异性。3种MAb能够区分蚜虫可传播的TuMV毒株1和非蚜虫可传播的毒株31，而4种MAb仅与毒株31发生反应。使用这些特异性MAb未发现两种毒株之间存在交叉反应。基于MAb抑制其他MAb反应的能力，抗体竞争试验表明，14种MAb识别病毒粒子上的6个不同表位；毒株1特异性的MAb识别2个表位，毒株31特异性的MAb也识别2个表位。其余2个表位是共同的。由于在两种毒株的外壳蛋白之间的6个氨基酸差异位于N端区域，毒株1或31特异性的MAb与两种毒株外壳蛋白N端区域的不同表位结合。
