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Production and characterization of monoclonal antibodies to plum pox virus and their use in differentiation of Mediterranean isolates.

作者信息

López-Moya J J, Sanz A, Cambra M, Gorris M T, Anaya C, Miguet J G, Cortés E, López-Abella D

机构信息

UEI Fitopatología, Centro de Investigaciones Biológicas, CSIC, Madrid, Spain.

出版信息

Arch Virol. 1994;135(3-4):293-304. doi: 10.1007/BF01310015.

DOI:10.1007/BF01310015
PMID:7526822
Abstract

Monoclonal antibodies (MAbs) specific to plum pox virus (PPV) were prepared by fusing myeloma cell lines to spleen cells of mice immunized with purified virus, including virus prepared with protease inhibitors to preserve the integrity of the coat protein (CP). The characterized MAbs could be used in ELISA to differentiate several Mediterranean PPV isolates differing in their geographical origin and CP size. At least seven antigenic sites could be established based on the recognition pattern and competition binding analysis, and the epitopes could be classified in three groups by Western blot analysis of intact and trypsin digested virus particles. By means of electron microscopy the epitopes could be seen to be located on the surface of the virus particles.

摘要

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本文引用的文献

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Monoclonal antibodies suitable for plum pox virus determination.适用于李痘病毒检测的单克隆抗体。
Hybridoma. 1993 Apr;12(2):215-20. doi: 10.1089/hyb.1993.12.215.
2
Characterization of epitopes recognized by monoclonal antibodies to aphid transmissible and non-transmissible strains of turnip mosaic virus.对芜菁花叶病毒蚜虫传播和非传播株系单克隆抗体所识别表位的特性分析
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Monoclonal antibodies define eight independent antigenic regions on the bovine leukemia virus (BLV) envelope glycoprotein gp51.单克隆抗体可确定牛白血病病毒（BLV）包膜糖蛋白gp51上的八个独立抗原区域。
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Monoclonal antibodies specific for African swine fever virus proteins.针对非洲猪瘟病毒蛋白的单克隆抗体。
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Dot hybridization detection of plum pox virus using 32P-labeled RNA probes representing non-structural viral protein genes.使用代表病毒非结构蛋白基因的32P标记RNA探针进行李痘病毒的斑点杂交检测。
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10
A PCR membrane spot assay for the detection of plum pox virus RNA in bark of infected trees.
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