Cai W Q, Bodin P, Loesch A, Sexton A, Burnstock G
Department of Anatomy and Developmental Biology, University College London, UK.
J Vasc Res. 1993 Nov-Dec;30(6):348-55. doi: 10.1159/000159017.
Endothelial cells of human umbilical vein and artery, both in situ and in culture, were examined ultrastructurally and at the light-microscope level using either the pre-embedding peroxidase-antiperoxidase or avidin-biotin peroxidase complex immunostaining techniques. Vasoactive intestinal polypeptide (VIP), substance P (SP), calcitonin gene-related peptide (CGRP) and arginine-vasopressin (AVP) immunoreactivity were localized in subpopulations of endothelial cells of the term umbilical vein and artery. The percentage of VIP-, SP-, CGRP- and AVP-immunoreactive cells in the umbilical vein was 12, 10, 11, and 7.5%, respectively, out of a total of 5,364 cells (from 15 umbilical cords) examined. The artery contained fewer VIP-, SP-, and CGRP-immunoreactive cells, but more AVP-immunoreactive cells, than the vein. In conclusion, subpopulations of endothelial cells in the human umbilical vein and artery contain the neuropeptides VIP, SP, CGRP and AVP, although their physiological roles are not yet known.
采用包埋前过氧化物酶-抗过氧化物酶或抗生物素蛋白-生物素过氧化物酶复合物免疫染色技术，在超微结构和光镜水平上对原位及培养的人脐静脉和动脉内皮细胞进行了检查。血管活性肠肽（VIP）、P物质（SP）、降钙素基因相关肽（CGRP）和精氨酸加压素（AVP）免疫反应性定位于足月脐静脉和动脉内皮细胞亚群中。在所检查的总共5364个细胞（来自15条脐带）中，脐静脉中VIP、SP、CGRP和AVP免疫反应性细胞的百分比分别为12%、10%、11%和7.5%。动脉中VIP、SP和CGRP免疫反应性细胞比静脉少，但AVP免疫反应性细胞比静脉多。总之，人脐静脉和动脉中的内皮细胞亚群含有神经肽VIP、SP、CGRP和AVP，尽管它们的生理作用尚不清楚。
