Ohya K, Ishige N, Mataki S, Ogura H
Department of Pharmacology, Faculty of Dentistry, Tokyo Medical and Dental University, Japan.
Nihon Yakurigaku Zasshi. 1993 Nov;102(5):323-31. doi: 10.1254/fpj.102.323.
We investigated the effect of microtubule inhibitors on the amelogenesis in rat incisor enamel at the maturation stage by the glyoxal bis (2-hydroxyanil) (GBHA) staining method. Several red stripes stained with GBHA appeared on the maturation enamel surface of control rats. Colchicine injection (1.3 mg/kg, s.c.) disarranged the GBHA staining stripes and increased the staining area. The ratio of the GBHA staining area to the maturation enamel surface was about 25% in the control, but this value increased about 60% at 8 and 24 hr after the colchicine injection. Lumicolchicine, which does not have the ability to disrupt microtubules, did not cause any significant changes in the enamel surface. The incorporation of 45Ca to the maturation enamel was also reduced by colchicine. Since the hypocalcemic action of microtubule inhibitors may be related to the change of the enamel surface, other drugs, sodium salicylate and sodium fluoride, that have a hypocalcemic action were tested. The staining pattern was not altered by these drugs. Therefore, the hypocalcemic action was independent of the changes of the GBHA staining pattern. These results indicated that the disruption of microtubules in the ameloblasts inhibited calcium movement in the maturation enamel, resulting in the disarrangement of the GBHA staining pattern.
我们通过乙二醛双（2-羟基苯胺）（GBHA）染色法研究了微管抑制剂对大鼠切牙釉质成熟阶段釉质形成的影响。在对照大鼠的成熟釉质表面出现了几条被GBHA染色的红色条纹。注射秋水仙碱（1.3mg/kg，皮下注射）使GBHA染色条纹紊乱，并增加了染色面积。对照中GBHA染色面积与成熟釉质表面的比例约为25%，但在注射秋水仙碱后8小时和24小时，该值增加了约60%。无破坏微管能力的光秋水仙碱未引起釉质表面任何显著变化。秋水仙碱也减少了45Ca向成熟釉质中的掺入。由于微管抑制剂的降钙作用可能与釉质表面的变化有关，因此对其他具有降钙作用的药物水杨酸钠和氟化钠进行了测试。这些药物未改变染色模式。因此，降钙作用与GBHA染色模式的变化无关。这些结果表明，成釉细胞中微管的破坏抑制了成熟釉质中的钙移动，导致GBHA染色模式紊乱。
