Gauvin D V, Briscoe R J, Goulden K L, Wojnicki F H, Russin R, Martin P R, Holloway F A
Department of Psychiatry and Behavioral Sciences, University of Oklahoma Health Sciences Center, Oklahoma City 73190-3000.
Alcohol Clin Exp Res. 1994 Dec;18(6):1398-405. doi: 10.1111/j.1530-0277.1994.tb01442.x.
Changes in sensitivity to ethanol's rate-decreasing effects on operant performance were examined in control rats and cohorts that received diet-induced or diet+pyrithiamine-induced thiamine deficiency. Seven groups of male Sprague-Dawley rats (12 rats/group) were trained in a 5-cycle lever-press operant task under a fixed-ratio 30 schedule of food reinforcement. Once trained to maintain consistent operant performance across all 5 cycles, each rat was tested with various doses of ethanol injected at the beginning of each time-out cycle. Each group of rats demonstrated equivalent saline baseline operant performance and ED50 for ethanol's rate-suppressing effects. Training sessions were suspended and rats received either a short- (9 days) or long-term (5-week) exposure to regular rat chow diet or thiamine-deficient diet, and received either saline or pyrithiamine injections in a 2 x 2 design. Three additional control groups were maintained on a regular rat chow diet and received supplemental injections of either thiamine+pyrithiamine injections, thiamine+saline injections, or saline+pyrithiamine injections. The controlled diet phase continued until the development of overt signs of thiamine deficiency, at which time thiamine supplements were administered for 4 days. In phase 3, all rats were retrained in the operant task and a second ethanol dose-effect function was generated. A history of thiamine deficiency and recovery failed to shift the behavioral dose-effect functions significantly for ethanol and their associated blood alcohol curves. Most interestingly, significant behavioral sensitization to ethanol's rate suppressant effects was demonstrated in the two control groups of rats receiving regular rat chow diet in combination with supplemental injections of thiamine and either saline or pyrithiamine.(ABSTRACT TRUNCATED AT 250 WORDS)
在对照大鼠以及接受饮食诱导或饮食加吡硫胺诱导的硫胺素缺乏的大鼠群体中，研究了对乙醇对操作性行为表现的速率降低作用的敏感性变化。将七组雄性斯普拉格-道利大鼠（每组12只）在固定比例30的食物强化时间表下进行5周期杠杆按压操作性任务训练。一旦训练至在所有5个周期中保持一致的操作性行为表现，每只大鼠在每个超时周期开始时接受不同剂量的乙醇注射进行测试。每组大鼠在生理盐水基线操作性行为表现以及乙醇速率抑制作用的半数有效剂量（ED50）方面表现相当。训练课程暂停，大鼠接受短期（9天）或长期（5周）的常规大鼠饲料饮食或硫胺素缺乏饮食，并按照2×2设计接受生理盐水或吡硫胺注射。另外三个对照组维持常规大鼠饲料饮食，并接受硫胺素加吡硫胺注射、硫胺素加生理盐水注射或生理盐水加吡硫胺注射的补充注射。对照饮食阶段持续至出现明显的硫胺素缺乏迹象，此时给予硫胺素补充剂4天。在第3阶段，所有大鼠在操作性任务中重新训练，并生成第二个乙醇剂量效应函数。硫胺素缺乏和恢复的历史未能显著改变乙醇的行为剂量效应函数及其相关的血醇曲线。最有趣的是，在接受常规大鼠饲料饮食并结合硫胺素和生理盐水或吡硫胺补充注射的两组对照大鼠中，显示出对乙醇速率抑制作用的显著行为敏化。（摘要截断于250字）
