Nakamura F, Suyama K
Laboratory of Molecular Technology of Animal Products, Faculty of Agriculture, Tohoku University, Sendai, Japan.
Anal Biochem. 1994 Nov 15;223(1):21-5. doi: 10.1006/abio.1994.1540.
An ion-paired high-performance liquid chromatographic method for the determination of aldosine, which is derived from the aldol crosslink of both elastin and collagen, is described. Aldosine was determined as the pyridine derivative 6-(3-pyridyl) piperidine-2-carboxylic acid, which was synthesized by oxidative decarboxylation of aldosine using iron(III). This method does not require radioisotopes to label the aldol crosslink. In addition, pyridinium crosslinks such as desmosines can also be measured.
本文描述了一种离子对高效液相色谱法，用于测定由弹性蛋白和胶原蛋白的醛醇交联反应产生的醛糖素。醛糖素通过使用铁（III）对醛糖素进行氧化脱羧反应合成的吡啶衍生物6-(3-吡啶基)哌啶-2-羧酸来测定。该方法不需要放射性同位素来标记醛醇交联反应。此外，诸如锁链素等吡啶鎓交联物也可以进行测定。
