Institut fur Pharmakologie, Universitatsklinikum Essen, Universitat Duisburg-Essen, Essen, Deutschland.
Dermatology. 2010;221(3):219-28. doi: 10.1159/000318905.
Over the past decade injectable hyaluronan (HA) formulations have been widely used to decrease the visibility of skin aging. However, little basic research has been performed to address their effect on dermal skin fibroblasts.
The aim of the present study was to investigate whether human skin fibroblasts are affected by exogenous non-cross-linked HA with respect to proliferation, migration and extracellular matrix composition.
The effect of a non-cross-linked HA on proliferation, migration and gene expression of human dermal fibroblasts was determined. Furthermore, affinity histochemistry of pericellular HA was performed.
Proliferation was significantly stimulated by HA whereas migration was not affected. Importantly, exogenous HA was incorporated into fine HA filaments of the pericellular fibroblast matrix.
This is the first evidence that a HA formulation used in humans for cosmetic reasons stimulates fibroblast proliferat
在过去的十年中，注射用透明质酸（HA）制剂被广泛用于减少皮肤衰老的可见度。然而，几乎没有进行基础研究来解决它们对真皮皮肤成纤维细胞的影响。
本研究旨在探讨外源性非交联 HA 是否会影响人皮肤成纤维细胞的增殖、迁移和细胞外基质组成。
确定非交联 HA 对人真皮成纤维细胞增殖、迁移和基因表达的影响。此外，还进行了细胞周 HA 的亲和组织化学研究。
HA 显著刺激增殖，而迁移不受影响。重要的是，外源性 HA 被纳入细胞周成纤维细胞基质中的精细 HA 纤维中。
这是第一个证据表明，用于美容目的的 HA 制剂刺激成纤维细胞增殖。
