Atkins D, Arndt G M, Izant J G
R.W. Johnson Pharmaceutical Research Institute, Sydney, NSW, Australia.
Biol Chem Hoppe Seyler. 1994 Nov;375(11):721-9. doi: 10.1515/bchm3.1994.375.11.721.
The use of antisense and ribozyme RNA to modulate gene expression is emerging as an effective genetic technique. A compilation of successful antisense gene suppression experiments reveals the absence of reports on the use of the yeast Saccharomyces cerevisiae as a host. We examine the field of antisense and ribozyme use in S. cerevisiae and discuss that this result is not due to any lack of attempts and may reflect unique features of S. cerevisiae biology. In an attempt to learn from cellular RNA physiology we review evidence for naturally occurring antisense RNA regulation. Although there are many examples of well characterised overlapping RNA transcripts there is, as yet, no clear evidence suggesting complementary RNA-dependent gene regulation in S. cerevisiae. The application of artificial antisense and ribozyme genes is then discussed with an emphasis on the role of yeast as a model system for the systematic and genetic analysis of antisense and ribozyme RNA function. In addition, potential reasons for the lack of attempts to use antisense or ribozyme genes to create pseudogenetic mutants are considered. We conclude that the application of successful antisense and ribozyme strategies in yeast may have to address features of S. cerevisiae RNA biology and offer experimental approaches that may identify some of these features.
利用反义RNA和核酶RNA来调控基因表达正逐渐成为一种有效的遗传技术。对成功的反义基因抑制实验的汇总表明，尚无关于使用酿酒酵母作为宿主的报道。我们研究了在酿酒酵母中使用反义RNA和核酶的领域，并讨论了这一结果并非由于缺乏尝试，而是可能反映了酿酒酵母生物学的独特特征。为了从细胞RNA生理学中汲取经验，我们回顾了天然存在的反义RNA调控的证据。尽管有许多特征明确的重叠RNA转录本的例子，但目前尚无明确证据表明酿酒酵母中存在互补RNA依赖性基因调控。随后讨论了人工反义RNA和核酶基因的应用，重点强调了酵母作为反义RNA和核酶RNA功能的系统和遗传分析模型系统的作用。此外，还考虑了缺乏使用反义或核酶基因来创建假基因突变体尝试的潜在原因。我们得出结论，在酵母中应用成功的反义RNA和核酶策略可能必须考虑酿酒酵母RNA生物学的特征，并提供可能识别其中一些特征的实验方法。
