Xu Y H, Lu Z Y, Ihler G M
Department of Medical Biochemistry and Genetics, College of Medicine, Texas A&M University Health Science Center, College Station 77843-1114.
Biochim Biophys Acta. 1995 Mar 22;1234(2):173-83. doi: 10.1016/0005-2736(94)00271-p.
A factor capable of deforming erythrocyte membranes, found in the culture supernatants of Bartonella bacilliformis, was purified 1840-fold using hydrophobic, ion exchange and gel exclusion chromatography. The final fractions contained a single detectable polypeptide species, referred to as deformin, having a molecular weight of 67000 by SDS-PAGE and a native molecular weight of 130,000 by gel exclusion chromatography or velocity sedimentation in a glycerol gradient. Erythrocytes treated with deformin acquire trenches, indentations, and invaginations which could be reversed by vanadate, dilauroylphosphatidylcholine (DLPC), or by raising the internal Ca2+ concentrations with the inophore A23187. Internal vacuoles also form. Erythrocytes treated with trypsin or neuraminidase are much more sensitive to deformin than untreated erythrocytes; erythrocytes treated with phospholipase D are less sensitive to deformin. This protein may play a role in causing the severe anemia which can result as a consequence of infection by B. bacilliformis.
在杆状巴尔通体培养上清液中发现的一种能够使红细胞膜变形的因子，通过疏水、离子交换和凝胶排阻色谱法进行了1840倍的纯化。最终馏分含有单一可检测的多肽物种，称为变形素，通过SDS-PAGE测定其分子量为67000，通过凝胶排阻色谱法或在甘油梯度中的速度沉降法测定其天然分子量为130000。用变形素处理的红细胞会出现沟槽、凹陷和内陷，这些可被钒酸盐、二月桂酰磷脂酰胆碱（DLPC）或用离子载体A23187提高内部Ca2+浓度所逆转。内部液泡也会形成。用胰蛋白酶或神经氨酸酶处理的红细胞比未处理的红细胞对变形素更敏感；用磷脂酶D处理的红细胞对变形素不太敏感。这种蛋白质可能在导致由杆状巴尔通体感染引起的严重贫血中起作用。
