Mernaugh G, Ihler G M
Department of Medical Biochemistry & Genetics, Texas A&M College of Medicine, College Station 77843.
Infect Immun. 1992 Mar;60(3):937-43. doi: 10.1128/iai.60.3.937-943.1992.
Bartonella bacilliformis, a hemotropic bacterium and the causative agent of the human disease bartonellosis, when incubated in a tryptone-based medium produces an extracellular factor, termed deformation factor (DF), which induces extensive indentations and trenches in trypsinized erythrocyte membranes. The factor is stable during storage at 4 degrees C. It can be inactivated by proteases or brief heating to 70 to 80 degrees C, can be precipitated by ammonium sulfate, is nondialyzable, and is retained by membranes with a 30,000-molecular-weight cutoff. These properties suggest that DF is probably a protein. Incubation of erythrocytes with phospholipase D renders them resistant to deformation by DF.
杆菌状巴尔通体是一种嗜血性细菌，也是人类疾病巴尔通体病的病原体。当它在基于胰蛋白胨的培养基中培养时，会产生一种细胞外因子，称为变形因子（DF），该因子会在胰蛋白酶处理过的红细胞膜上诱导出大量凹陷和沟槽。该因子在4℃储存期间稳定。它可被蛋白酶或短暂加热至70至80℃灭活，可被硫酸铵沉淀，不可透析，并且可被截留分子量为30,000的膜保留。这些特性表明DF可能是一种蛋白质。用磷脂酶D孵育红细胞可使其对DF诱导的变形产生抗性。
