Hiraga M, Nunoya T, Otaki Y, Tajima M, Saito T, Nakamura T
Nippon Institute for Biological Science, Tokyo, Japan.
J Vet Med Sci. 1994 Dec;56(6):1057-63. doi: 10.1292/jvms.56.1057.
The pathogenesis of highly virulent infectious bursal disease (IBD) virus (IBDV) infection was studied using 6-week-old intact and 5-week-old bursectomized chickens inoculated with highly virulent strain 90-11 or reference strain I. Chickens inoculated with 10(0.7) EID50 of strain 90-11 showed neither clinical signs nor lesions during the 4-day observation period. In contrast, birds inoculated with 10(2.7) or 10(4.7) EID50 developed severe clinical IBD, as well as gross and histologic lesions, typical of IBD, and produced IBDV antigen demonstrable by immunostaining in the bursa of Fabricius (BF), thymus, spleen and bone marrow from day 2 post-inoculation (PI) onwards. The antigen was also detected by the agar-gel precipitation and latex microsphere agglutination tests in a bursal suspension of these birds from day 2 or day 3 PI on. Birds inoculated with 10(6.1) EID50 of strain I developed only slight clinical signs at day 4 PI. Their lesion- and antigen-scores in the BF were almost the same as those in virulent strain-infected chickens, but lesion- and antigen-scores in the other organs were negligible. Bursectomized chickens inoculated with strain 90-11 did not develop clinical IBD despite the presence of infection that was evidenced by histologic lesions in the thymus and spleen as well as IBDV antigen demonstrable by immunostaining in these organs.
使用6周龄完整鸡和5周龄法氏囊切除鸡，接种高毒力90 - 11株或参考株I，研究高毒力传染性法氏囊病（IBD）病毒（IBDV）感染的发病机制。接种10(0.7) EID50 90 - 11株的鸡在4天观察期内既无临床症状也无病变。相比之下，接种10(2.7)或10(4.7) EID50的鸡出现严重的临床IBD，以及IBD典型的大体和组织学病变，并从接种后（PI）第2天起在法氏囊（BF）、胸腺、脾脏和骨髓中产生可通过免疫染色检测到的IBDV抗原。从接种后第2天或第3天起，在这些鸡的法氏囊悬液中也通过琼脂凝胶沉淀和乳胶微球凝集试验检测到该抗原。接种10(6.1) EID50 I株的鸡在接种后第4天仅出现轻微临床症状。它们在BF中的病变和抗原评分与感染强毒株的鸡几乎相同，但其他器官中的病变和抗原评分可忽略不计。接种90 - 11株的法氏囊切除鸡尽管存在感染（胸腺和脾脏的组织学病变以及这些器官中通过免疫染色可检测到的IBDV抗原证明了感染的存在），但未发生临床IBD。
