Mittal S, Cohen A, Maysinger D
Department of Pharmacology and Therapeutics, McGill University, Montreal, Quebec, Canada.
Neuroreport. 1994 Dec 20;5(18):2577-82. doi: 10.1097/00001756-199412000-00043.
The principal objective of these studies was to microencapsulate brain derived neurotrophic factor (BDNF) and to test its biological effects in vitro. Two types of microspheres with BDNF were prepared using biodegradable polymers: chitosan and poly-(L-lactic acid):poly-(L-glycolic acid). Depending on the procedure, sizes ranged from 1 micron to 1 mm as determined by an image analysis system. The biological assays confirmed that the released BDNF remains biologically active in vitro. Microencapsulation of BDNF and other trophic factors may represent a viable alternative to currently employed delivery systems in vivo and holds potential for eventual therapy of neurodegenerative disorders, in particular for peripheral neuropathies and CNS disorders where supplementation of neurotrophins retards or even prevents neural degeneration.
这些研究的主要目的是将脑源性神经营养因子（BDNF）微囊化，并在体外测试其生物学效应。使用可生物降解聚合物壳聚糖和聚（L-乳酸）：聚（L-乙醇酸）制备了两种含有BDNF的微球。根据制备方法，通过图像分析系统测定，微球尺寸范围为1微米至1毫米。生物学检测证实，释放的BDNF在体外仍保持生物活性。BDNF和其他营养因子的微囊化可能是目前体内应用的递送系统的一种可行替代方案，并且具有最终治疗神经退行性疾病的潜力，特别是对于补充神经营养因子可延缓甚至预防神经变性的周围神经病变和中枢神经系统疾病。
