3-氨基吡啶甲酸作为生物聚合物激光解吸质谱分析的基质。

3-Aminopicolinic acid as a matrix for laser desorption mass spectrometry of biopolymers.

作者信息

Taranenko N I, Tang K, Allman S L, Ch'ang L Y, Chen C H

机构信息

Oak Ridge National Laboratory, Tennessee 37831-6378.

出版信息

Rapid Commun Mass Spectrom. 1994 Dec;8(12):1001-6. doi: 10.1002/rcm.1290081219.

DOI:10.1002/rcm.1290081219

Abstract

3-Aminopicolinic acid (3-APA) was tested and found to be a useful matrix for matrix-assisted laser desorption/ionization of DNA and protein. Single-stranded DNA segments of 150-mer and double-stranded DNA of 246 base pairs were successfully detected by using 3-APA as an ultraviolet-absorbing matrix in a linear time-of-flight mass spectrometer. In the case of the double-stranded DNA, only parent ions corresponding to single-stranded DNA were observed. The comparison with 3-hydroxypicolinic acid and picolinic acid matrices is discussed.

摘要

对3-氨基吡啶甲酸（3-APA）进行了测试，发现它是用于DNA和蛋白质的基质辅助激光解吸/电离的一种有用基质。在飞行时间线性质谱仪中，以3-APA作为紫外吸收基质，成功检测到了150个碱基的单链DNA片段和246个碱基对的双链DNA。对于双链DNA的情况，仅观察到了与单链DNA相对应的母离子。文中还讨论了与3-羟基吡啶甲酸和吡啶甲酸基质的比较。

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