Tang K, Taranenko N I, Allman S L, Cháng L Y, Chen C H
Oak Ridge National Laboratory, Chemical Physics Section, Tennessee 37831-6378.
Rapid Commun Mass Spectrom. 1994 Sep;8(9):727-30. doi: 10.1002/rcm.1290080913.
We report the first detection of DNA segments as large as 500 nucleotides by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, using picolinic acid and 3-hydroxypicolinic acid mixtures as desorption matrices. The successful detection of 500-nucleotide DNA indicates that laser mass spectrometry is now emerging as a new biotechnology tool for DNA-related research. It should be possible to utilize fast detection of large DNA segments by laser mass spectrometry for rapid human genome sequencing.
我们报告了首次使用吡啶甲酸和3-羟基吡啶甲酸混合物作为解吸基质，通过基质辅助激光解吸/电离（MALDI）质谱法检测到长达500个核苷酸的DNA片段。成功检测到500个核苷酸的DNA表明激光质谱法正在成为一种用于DNA相关研究的新型生物技术工具。利用激光质谱法快速检测大的DNA片段进行快速人类基因组测序应该是可行的。
