使用质谱法进行梯状条带检测来测序DNA。
Sequencing DNA using mass spectrometry for ladder detection.
作者信息
Taranenko N I, Allman S L, Golovlev V V, Taranenko N V, Isola N R, Chen C H
机构信息
Life Science Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6378, USA andYale University Medical Center, New Haven, CT, USA.
出版信息
Nucleic Acids Res. 1998 May 15;26(10):2488-90. doi: 10.1093/nar/26.10.2488.
Abstract
Sequencing of DNA fragments of 130 and 200 bp using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for DNA ladder detection was demonstrated. With further improvement in mass resolution and detection sensitivity, mass spectrometry shows great promise for routine DNA sequencing in the future.
摘要
已证明使用基质辅助激光解吸/电离飞行时间质谱法对130和200 bp的DNA片段进行测序以检测DNA阶梯。随着质量分辨率和检测灵敏度的进一步提高，质谱法在未来的常规DNA测序中显示出巨大的前景。
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