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使用质谱法进行梯状条带检测来测序DNA。

Sequencing DNA using mass spectrometry for ladder detection.

作者信息

Taranenko N I, Allman S L, Golovlev V V, Taranenko N V, Isola N R, Chen C H

机构信息

Life Science Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6378, USA andYale University Medical Center, New Haven, CT, USA.

出版信息

Nucleic Acids Res. 1998 May 15;26(10):2488-90. doi: 10.1093/nar/26.10.2488.

DOI:10.1093/nar/26.10.2488
PMID:9580704
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC147544/
Abstract

Sequencing of DNA fragments of 130 and 200 bp using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for DNA ladder detection was demonstrated. With further improvement in mass resolution and detection sensitivity, mass spectrometry shows great promise for routine DNA sequencing in the future.

摘要

已证明使用基质辅助激光解吸/电离飞行时间质谱法对130和200 bp的DNA片段进行测序以检测DNA阶梯。随着质量分辨率和检测灵敏度的进一步提高，质谱法在未来的常规DNA测序中显示出巨大的前景。

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本文引用的文献

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A strategy for rapid and efficient DNA sequencing by mass spectrometry.一种通过质谱法进行快速高效DNA测序的策略。
Nat Biotechnol. 1996 Sep;14(9):1123-8. doi: 10.1038/nbt0996-1123.
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Sequence from spectrometry: a realistic prospect?
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Matrix-assisted laser desorption/ionization for sequencing single-stranded and double-stranded DNA.用于单链和双链DNA测序的基质辅助激光解吸/电离技术。
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Analyzing sequencing reactions from bacteriophage M13 by matrix-assisted laser desorption/ionization mass spectrometry.通过基质辅助激光解吸/电离质谱分析噬菌体M13的测序反应。
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6
Mass resolution improvement by incorporation of pulsed ion extraction in a matrix-assisted laser desorption/ionization linear time-of-flight mass spectrometer.通过在基质辅助激光解吸/电离线性飞行时间质谱仪中引入脉冲离子提取来提高质量分辨率。
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High-resolution matrix-assisted laser desorption/ionization in a linear time-of-flight mass spectrometer.线性飞行时间质谱仪中的高分辨率基质辅助激光解吸/电离
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DNA sequence determination by hybridization: a strategy for efficient large-scale sequencing.通过杂交进行DNA序列测定：一种高效大规模测序的策略。
Science. 1993 Jun 11;260(5114):1649-52. doi: 10.1126/science.8503011.
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Matrix-assisted laser desorption time-of-flight mass spectrometry of oligonucleotides using 3-hydroxypicolinic acid as an ultraviolet-sensitive matrix.使用3-羟基吡啶甲酸作为紫外线敏感基质的寡核苷酸基质辅助激光解吸飞行时间质谱分析。
Rapid Commun Mass Spectrom. 1993 Feb;7(2):142-6. doi: 10.1002/rcm.1290070206.