Panayotacopoulou M T, Raadsheer F C, Swaab D F
Department of Psychiatry, University of Athens, Eginition Hospital, Greece.
Brain Res Dev Brain Res. 1994 Nov 18;83(1):59-66. doi: 10.1016/0165-3806(94)90179-1.
In the developing and adult human paraventricular (PVN) and supraoptic (SON) nucleus, a large proportion of neurons contains the catecholamine-synthesizing enzyme tyrosine hydroxylase (TH). In the present study we investigated the possible colocalization of TH with oxytocin (OXT) or vasopressin (VP) in the adult and neonatal PVN and SON. Adjacent paraffin sections were incubated simultaneously with two antibodies: a polyclonal against TH and a monoclonal against OXT or VP and stained with a double peroxidase-antiperoxidase/alkaline phosphatase method. We observed that TH-immunoreactive(IR) perikarya in the human PVN and SON were also positive for OXT or VP. A clear difference between the neonates and adult cases of our sample was observed in the proportion of TH-IR neurons that colocalize OXT or VP. In the neonates the majority of the TH-IR perikarya was also stained for VP, while only few TH-IR neurons were also positive for OXT. The opposite was observed in the adults, where the majority of the double-stained TH-IR neurons colocalizes OXT while only few TH-IR perikarya appear to contain VP. Our study establishes the colocalization of TH with OXT or VP in the adult and neonatal PVN and SON and indicates that antemortem factors such as perinatal hypoxia might increase TH-immunoreactivity of the VP neurons in man.
在发育中的和成年人类的室旁核（PVN）和视上核（SON）中，很大一部分神经元含有儿茶酚胺合成酶酪氨酸羟化酶（TH）。在本研究中，我们调查了成年和新生的PVN及SON中TH与催产素（OXT）或血管加压素（VP）可能的共定位情况。相邻的石蜡切片同时用两种抗体孵育：一种是抗TH的多克隆抗体，另一种是抗OXT或VP的单克隆抗体，并用双过氧化物酶 - 抗过氧化物酶/碱性磷酸酶方法染色。我们观察到人类PVN和SON中TH免疫反应性（IR）的胞体对OXT或VP也呈阳性。在我们的样本中，新生儿和成年病例之间在共定位OXT或VP的TH - IR神经元比例上存在明显差异。在新生儿中，大多数TH - IR胞体也被VP染色，而只有少数TH - IR神经元对OXT呈阳性。在成年人中观察到相反的情况，大多数双重染色的TH - IR神经元共定位OXT，而只有少数TH - IR胞体似乎含有VP。我们的研究确定了成年和新生的PVN及SON中TH与OXT或VP的共定位，并表明诸如围产期缺氧等生前因素可能会增加人类VP神经元的TH免疫反应性。
