Lacki J K, Klama K, Samborski W, Mackiewicz S H, Muller W
Hochrhein Institut fur Rheumaforschung und Rheumapravention, Bad Sackingen, Germany.
Clin Rheumatol. 1994 Dec;13(4):598-604. doi: 10.1007/BF02243001.
Microheterogeneity of alpha-1-acid glycoprotein was studied using affinity immunoelectrophoresis with concanavalin A as a ligand in the samples of serum and synovial fluid obtained at the same time from 22 patients with rheumatoid arthritis. Individuals with intercurrent infection or other illnesses were excluded from the study. The results were expressed as reactivity coefficient (RC). Disease activity was evaluated by Mallya-Mace Activity Score, Lansbury Joint Index and laboratory tests. In most of the studied samples the glycosylation pattern was similar, composed of a nonreactive variant and 2 reactive (the first and the second) with Con A variants. In seven samples of synovial fluid an extra third peak representative of the strongly reactive one with Con A fraction was observed. It was the cause of the remarkable elevation of AGP-RC. Moreover, the level of IgM and IgA RF was higher in the synovial fluid derived from these patients--with the presence of the third peak in AFF-EP with Con A--than in those without the considered fraction.
利用伴刀豆球蛋白A作为配体的亲和免疫电泳，对22例类风湿关节炎患者同时采集的血清和滑液样本中的α-1-酸性糖蛋白的微观异质性进行了研究。研究排除了并发感染或患有其他疾病的个体。结果以反应系数（RC）表示。通过Mallya-Mace活动评分、Lansbury关节指数和实验室检查评估疾病活动度。在大多数研究样本中，糖基化模式相似，由一个无反应性变体和2个与伴刀豆球蛋白A反应性变体（第一个和第二个）组成。在7个滑液样本中，观察到一个额外的第三个峰，代表与伴刀豆球蛋白A部分强反应性的峰。这是AGP-RC显著升高的原因。此外，在这些患者的滑液中——在伴刀豆球蛋白A的亲和免疫电泳中有第三个峰——IgM和IgA类风湿因子水平高于没有该部分的患者。
