Pinto H C, Portela-Gomes G M, Grimelius L, Kohnert K D, de Sousa J C, Albuquerque M A
Department of Medicine II, University Hospital of Santa Maria, Lisbon, Portugal.
Gastroenterology. 1995 Apr;108(4):967-74. doi: 10.1016/0016-5085(95)90191-4.
BACKGROUND/AIMS: Genetically diabetic (db/db) mice are a model for non-insulin-dependent diabetes in humans. The gastrointestinal tracts in 12-week-old db/db and nondiabetic control (db/+) mice were studied with particular emphasis on the endocrine cells.
Immunocytochemical and quantification techniques were used to localize and determine the number of cells containing serotonin and various regulatory peptides.
In the antrum, the gastrin- and serotonin-immunoreactive cells were increased in number. In the large intestine, the enteroglucagon and the peptide tyrosine-immunoreactive cells were increased in number, whereas there were fewer serotonin-immunoreactive cells. There were also fewer somatostatin-immunoreactive cells in most gastrointestinal regions. In diabetic mice, the intestine was longer and its mucosa thicker than in control mice.
The results indicate that the genetic diabetic (db/db) condition exerts a significant influence on the gastrointestinal tract and on the endocrine cell systems studied. The observed alterations may reflect the effect of indirect factors rather than the diabetes per se.
背景/目的：遗传性糖尿病（db/db）小鼠是人类非胰岛素依赖型糖尿病的一种模型。对12周龄的db/db小鼠和非糖尿病对照（db/+）小鼠的胃肠道进行了研究，特别关注内分泌细胞。
采用免疫细胞化学和定量技术来定位并确定含5-羟色胺和各种调节肽的细胞数量。
在胃窦部，胃泌素和5-羟色胺免疫反应性细胞数量增加。在大肠，肠高血糖素和肽酪氨酸免疫反应性细胞数量增加，而5-羟色胺免疫反应性细胞较少。在大多数胃肠道区域，生长抑素免疫反应性细胞也较少。糖尿病小鼠的肠道比对照小鼠更长，其黏膜更厚。
结果表明遗传性糖尿病（db/db）状态对胃肠道及所研究的内分泌细胞系统有显著影响。观察到的改变可能反映了间接因素的作用而非糖尿病本身的作用。
