Fonseca I, Costa Rosa J, Félix A, Therkildsen M H, Mandel U, Soares J
Departamento de Patologia Morfológica, Instituto Português de Oncologia de Francisco Gentil, Lisboa.
Histopathology. 1994 Dec;25(6):537-43. doi: 10.1111/j.1365-2559.1994.tb01372.x.
Thirty-two cases of mucoepidermoid carcinoma of the salivary glands were studied in order to characterize the expression of simple mucin-type carbohydrate antigens T, Tn and sialosyl-Tn and to evaluate its implication for tumour histogenesis. Monoclonal antibodies of known specificity were used on formalin-fixed, paraffin-embedded tissue, and the expression of these antigens was studied in each of the three cell types (mucous, intermediate and squamous) as well as in the secretory content of neoplastic lumina. Aberrant glycosylation of simple-mucin type antigens was found in all cell types, as compared with that of normal excretory duct cells of the salivary glands. The more 'primitive' antigens Tn and sialosyl-Tn were present in a high percentage of epidermoid and intermediate cells. Mucous cells and the intraluminal secretory content also expressed Tn in 57.7% of the cases. This contrasts with the absence of secretion of these simple mucin type carbohydrates by normal salivary gland cells. Mucin-producing cells did not express T antigen but only sialosyl-T, in contrast to 57.1% and 56.3% respectively of the epidermoid and intermediate cell types. T and sialosyl-T were also found in the secretory products of the neoplastic lumina in 11.5% and 53.6% of the cases, respectively. The distinctive glycosylation pattern between mucin-producing cells on the one hand and intermediate and squamous cells on the other does not contradict the common origin of the three cell types from the reserve cell of the salivary excretory duct, but favours the proposition that intermediate cells constitute a step in the differentiation pathway of epidermoid, but not of mucin-producing, cells.
为了明确单纯粘蛋白型碳水化合物抗原T、Tn和唾液酸化Tn的表达特征，并评估其对肿瘤组织发生的意义，对32例涎腺黏液表皮样癌进行了研究。使用已知特异性的单克隆抗体作用于福尔马林固定、石蜡包埋的组织，研究这些抗原在三种细胞类型（黏液细胞、中间细胞和鳞状细胞）以及肿瘤管腔内分泌成分中的表达。与涎腺正常排泄管细胞相比，所有细胞类型中均发现了单纯粘蛋白型抗原的异常糖基化。“原始”抗原Tn和唾液酸化Tn在高比例的表皮样细胞和中间细胞中存在。黏液细胞和管腔内分泌成分在57.7%的病例中也表达Tn。这与正常涎腺细胞不分泌这些单纯粘蛋白型碳水化合物形成对比。产生粘蛋白的细胞不表达T抗原，仅表达唾液酸化T，而表皮样细胞和中间细胞类型中分别有57.1%和56.3%表达T抗原。在11.5%和53.6%的病例中，肿瘤管腔的分泌产物中也分别发现了T和唾液酸化T。一方面产生粘蛋白的细胞与另一方面中间细胞和鳞状细胞之间独特的糖基化模式，并不与这三种细胞类型均起源于涎腺排泄管储备细胞相矛盾，而是支持中间细胞构成表皮样细胞而非产生粘蛋白细胞分化途径中的一个步骤这一观点。
