Therkildsen M H, Mandel U, Christensen M, Dabelsteen E
Department of Pathology Rigshospitalet, University of Copenhagen, Denmark.
Eur J Cancer B Oral Oncol. 1995 Nov;31B(6):361-7. doi: 10.1016/0964-1955(95)00044-5.
The simple mucin-type T (Thomsen-Friedenreich) antigen is a marker of carcinomas, and has been related to aggressiveness of malignant tumours. We studied the expression of T, sialosyl-T, A and H blood group antigens in salivary gland carcinomas. The aim was to study whether the tumours, based on the expression of these structures, could be divided into new diagnostic groups that may later show prognostic significance. Formalin fixed paraffin-embedded tissue sections from 77 salivary gland carcinomas of different histological types were studied using immunohistology and monoclonal antibodies (MAbs). Fresh frozen tissue was examined in 30 of the cases. Frozen sections were superior to paraffin sections in demonstrating T and H antigens. Aberrant glycosylation with accumulation of T (in cytoplasm) and sialosyl-T antigens (in cytoplasm, membrane and mucin) was found in all tumour types except acinic cell carcinomas. In carcinomas in pleomorphic adenomas (CinPA) the effect of fixation was minimal and T antigen location was different. In carcinomas with myoepithelial cell (MEC) participation, the MECs had retained a normal glycosylation pattern. H antigen was expressed in all tumour types, except acinic cell carcinomas and CinPA. A antigen was expressed in all tumour types from blood group A patients, except in CinPA. The expression of T, sialosyl-T, H and A antigens in relation to differentiation grade varied with tumour type in poorly differentiated areas. High and moderate differentiated areas were always stained, whereas poorly differentiated areas in some tumour types expressed T and sialosyl-T antigens and others did not. The accumulation versus lack of expression of the investigated structures in poorly differentiated areas of the tumours may be of prognostic significance.
简单粘蛋白型T（汤姆森 - 弗里德赖希）抗原是癌的标志物，并且与恶性肿瘤的侵袭性有关。我们研究了唾液腺癌中T、唾液酸化T、A和H血型抗原的表达。目的是研究基于这些结构的表达，肿瘤是否可以被分为新的诊断组，这些诊断组随后可能显示出预后意义。使用免疫组织化学和单克隆抗体（MAb）对77例不同组织学类型的唾液腺癌的福尔马林固定石蜡包埋组织切片进行了研究。其中30例病例检查了新鲜冷冻组织。冷冻切片在显示T和H抗原方面优于石蜡切片。除腺泡细胞癌外，在所有肿瘤类型中均发现了T（在细胞质中）和唾液酸化T抗原（在细胞质、细胞膜和粘蛋白中）的异常糖基化积累。在多形性腺瘤中的癌（CinPA）中，固定的影响最小，T抗原的定位不同。在有肌上皮细胞（MEC）参与的癌中，MEC保留了正常的糖基化模式。除腺泡细胞癌和CinPA外，所有肿瘤类型均表达H抗原。来自A型血患者的所有肿瘤类型中均表达A抗原，但CinPA除外。在低分化区域，T、唾液酸化T、H和A抗原的表达与分化程度的关系因肿瘤类型而异。高分化和中分化区域总是被染色，而某些肿瘤类型的低分化区域表达T和唾液酸化T抗原，而其他肿瘤类型则不表达。肿瘤低分化区域中所研究结构的积累与不表达情况可能具有预后意义。
