el-Sabban F, Fahim M A
Department of Physiology, Faculty of Medicine and Health Sciences, U.A.E. University, Al Ain.
Int J Biometeorol. 1995 Jan;38(2):92-7. doi: 10.1007/BF01270666.
The effect of local cerebral hyperthermia on responses of pial microvessels of the mouse was investigated. A set protocol was followed, involving the performance of a craniotomy on anaesthetized animals and using intravital microscope-television closed circuitry. Controlled hyperthermic exposure was applied regionally by heating the brain surface with irrigating artificial cerebrospinal fluid. Microvascular responses such as changes in diameter, thrombosis and embolism were monitored and video-taped observations were further viewed and analysed. When both brain surface and core body temperatures were kept at 37 degrees C, no changes in pial microvessels were noted. With core body temperature kept at 37 degrees C and at a brain surface temperature of 43.1 degrees C, passing emboli and arteriolar constriction were observed. A few minutes later, visible thrombosis was prevalent. Further spontaneous thrombo-embolic activity continued and at the end of a 50-min hyperthermic exposure, arterioles attained a constriction of 37%. Thrombus formation was sometimes massive enough to occlude fully the microvessel. The protocol followed in this study can be adopted to other small animal species and for a variety of experimental procedures involving hyperthermia and the pial microcirculation.
研究了局部脑热疗对小鼠软脑膜微血管反应的影响。遵循一套既定方案，包括对麻醉动物进行开颅手术并使用活体显微镜 - 电视闭路系统。通过用人工脑脊液冲洗加热脑表面来局部施加可控的热暴露。监测微血管反应，如直径变化、血栓形成和栓塞，并对录像观察结果进行进一步查看和分析。当脑表面温度和核心体温均保持在37℃时，未观察到软脑膜微血管有变化。当核心体温保持在37℃且脑表面温度为43.1℃时，观察到有栓子通过和小动脉收缩。几分钟后，可见血栓形成普遍。进一步的自发血栓栓塞活动持续存在，在50分钟的热暴露结束时，小动脉收缩达37%。血栓形成有时严重到足以完全阻塞微血管。本研究中遵循的方案可应用于其他小动物物种以及各种涉及热疗和软脑膜微循环的实验程序。
