Tohya K, Kimura M
Department of Anatomy, Kansai Shinkyu College, Kansai Academy of Medical Sciences, Osaka, Japan.
Histochemistry. 1994 Dec;102(6):445-50. doi: 10.1007/BF00269576.
We have developed and characterized three monoclonal antibodies (mAbs), which recognize the surface antigens of musk shrew B cells and T cells. About 30% of all lymph node cells reacted with the mAb ST1. Staining of frozen sections showed that ST1-positive cells were located in the primary lymphoid follicles of lymph nodes, and were absent in the thymus. mAbs ST4 and ST2 were also surface-reactive. The ST1-negative (about 60% of the total) lymph node cells reacted positively with ST4, and about 30% of these ST4-positive cells were recognized by ST2. The distribution of ST4-positive cells was shown by staining of lymph node sections to be identical to that of T cells reported in other species. Western blot analysis showed that the apparent molecular weights of the antigens recognized by ST1 and ST4 were 70000 and 74000, and 60000 and 64000, respectively. These findings suggest that the antigens detected by ST1, ST4, and ST2 are the musk shrew homologues of pan-B cells, pan-T cells, and T cell subsets, respectively. The three mABs may facilitate immunohistochemical analysis of the cellular immune response in this species.
我们已经开发并鉴定了三种单克隆抗体（mAb），它们可识别麝鼩B细胞和T细胞的表面抗原。所有淋巴结细胞中约30%与单克隆抗体ST1发生反应。冰冻切片染色显示，ST1阳性细胞位于淋巴结的初级淋巴滤泡中，而胸腺中不存在。单克隆抗体ST4和ST2也具有表面反应性。ST1阴性的淋巴结细胞（约占总数的60%）与ST4呈阳性反应，其中约30%的ST4阳性细胞可被ST2识别。淋巴结切片染色显示，ST4阳性细胞的分布与其他物种报道的T细胞分布相同。蛋白质印迹分析表明，ST1和ST4识别的抗原的表观分子量分别为70000和74000，以及60000和64000。这些发现表明，ST1、ST4和ST2检测到的抗原分别是泛B细胞、泛T细胞和T细胞亚群的麝鼩同源物。这三种单克隆抗体可能有助于对该物种的细胞免疫反应进行免疫组织化学分析。
