实验用麝鼩（Suncus murinus）外周和黏膜淋巴组织中淋巴细胞分布的免疫组织化学研究

Immunohistochemical studies on the distribution of lymphocytes in the peripheral and mucosal lymphoid tissues of laboratory musk shrews (Suncus murinus).

作者信息

Tohya K, Kimura M, Kakudo K, Kawamata J

机构信息

Department of Anatomy, Kansai Shinkyu College, Kansai Academy of Medical Science, Osaka, Japan.

出版信息

Dev Comp Immunol. 1992 Nov-Dec;16(6):473-83. doi: 10.1016/0145-305x(92)90031-7.

DOI:10.1016/0145-305x(92)90031-7

PMID:1473597

Abstract

We developed a new monoclonal antibody (mAb), named 1E9, which recognizes the immunoglobulin (Ig) M-like molecule of the insectivore laboratory animal, the suncus. This mAb was developed in order to investigate the distribution of surface Ig (sIg)-bearing lymphocytes in the lymphoid tissues of this animal. Immunohistochemically, the distribution of 1E9+ lymphocytes in the follicles of peripheral lymphoid tissues showed homology with such distribution in rodents. However, the lymphocytes in the follicles of the mucosal lymphoid tissues were not recognized by the mAb. These data suggest that from a comparative immunological viewpoint, our newly developed 1E9 mAb is useful for the characterization of sIg+ lymphocytes in the peripheral and mucosal lymphoid tissues of the suncus.

摘要

我们开发了一种名为1E9的新型单克隆抗体（mAb），它可识别食虫目实验动物——臭鼩的免疫球蛋白（Ig）M样分子。开发这种单克隆抗体是为了研究该动物淋巴组织中携带表面免疫球蛋白（sIg）的淋巴细胞的分布。免疫组织化学研究表明，外周淋巴组织滤泡中1E9阳性淋巴细胞的分布与啮齿动物中的此类分布具有同源性。然而，黏膜淋巴组织滤泡中的淋巴细胞未被该单克隆抗体识别。这些数据表明，从比较免疫学的角度来看，我们新开发的1E9单克隆抗体可用于表征臭鼩外周和黏膜淋巴组织中的sIg阳性淋巴细胞。