采用杂交法通过线性探针分析对丙型肝炎病毒分离株进行亚型分型。
Subtyping of hepatitis C virus isolates by a line probe assay using hybridization.
作者信息
Andonov A, Chaudhary R K
机构信息
Laboratory for Viral Hepatitis, Laboratory Centre for Disease Control, Health Canada, Ottawa, Ontario.
出版信息
J Clin Microbiol. 1995 Jan;33(1):254-6. doi: 10.1128/jcm.33.1.254-256.1995.
Abstract
A reverse hybridization test (Inno-LiPA HCV; Innogenetics, N.V., Zwijnaarde, Belgium) was used for typing hepatitis C virus. All 38 samples, typed by PCR with primers from core and NS5 genes, were also genotyped by this test. Of the samples, 33 (87%) had the same subtypes by both assays. The correlations between PCR and Inno-LiPA for individual types were 77% for type I (1a), 90% for type II (1b), 100% for type III (2a), 100% for type IV (2b), and 100% for type V (3a). One of the type III (2a) samples also reacted with type I (1a) probes in the Inno-LiPA test.
摘要
采用反向杂交试验（Inno-LiPA HCV；比利时兹温纳尔德的Innogenetics公司）对丙型肝炎病毒进行分型。通过使用来自核心基因和NS5基因的引物进行PCR分型的所有38个样本，也采用该试验进行基因分型。在这些样本中，两种检测方法得出相同亚型的有33个（87%）。PCR和Inno-LiPA针对各型的相关性分别为：I型（1a）77%，II型（1b）90%，III型（2a）100%，IV型（2b）100%，V型（3a）100%。在Inno-LiPA试验中，1个III型（2a）样本也与I型（1a）探针发生反应。
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