来自活动性白塞病患者的表达γδ受体的T细胞的表型和功能特征

Phenotype and functional profile of T cells expressing gamma delta receptor from patients with active Behçet's disease.

作者信息

Hamzaoui K, Hamzaoui A, Hentati F, Kahan A, Ayed K, Chabbou A, Ben Hamida M, Hamza M

机构信息

Immunology Laboratory, Medical School, University of Tunis.

出版信息

J Rheumatol. 1994 Dec;21(12):2301-6.

PMID:7699633

Abstract

OBJECTIVE

Our aim was to investigate the TCR gamma delta+ subset in Behçet's disease (BD) inflammatory sites, which better reflects changes associated with the pathologic process than peripheral blood.

METHODS

Forty-five patients with active BD, 10 patients with recurrent aphthous ulcers, 12 patients with rheumatoid arthritis, 5 patients with noninflammatory neurologic diseases and 15 healthy individuals were studied. Three monoclonal antibodies TCR delta 1, BB3, and A13 were used to assess the percentage of TCR gamma delta+ in peripheral blood mononuclear cells (PBMC), in bronchoalveolar lavage and cerebrospinal fluid (CSF). CD11a/CD18 was used to study adhesion molecules. TCR gamma delta+ cells isolated by immunomagnetic separation were tested for cytolytic activity against K562 target cells after interleukin 2 stimulation.

RESULTS

The PBMC TCR gamma delta BB3+ subset was significantly increased in BD. In BD inflammatory sites, TCR gamma delta+ cells were also present, composed mainly of A13+ cells from these sites also expressed CD11a marker. TCR gamma delta+ cells from inflammatory sites displayed a higher cytotoxic activity than controls, mediated by the A13+ subset.

CONCLUSION

The accumulation of cytotoxic TCR gamma delta+ cells at the sites of inflammation suggests their involvement in the local injury process.

摘要

目的

我们的目的是研究白塞病（BD）炎症部位的TCRγδ⁺亚群，该亚群比外周血能更好地反映与病理过程相关的变化。

方法

对45例活动期BD患者、10例复发性阿弗他溃疡患者、12例类风湿关节炎患者、5例非炎性神经系统疾病患者和15名健康个体进行了研究。使用三种单克隆抗体TCRδ1、BB3和A13来评估外周血单个核细胞（PBMC）、支气管肺泡灌洗液和脑脊液（CSF）中TCRγδ⁺的百分比。使用CD11a/CD18研究黏附分子。通过免疫磁珠分离法分离出的TCRγδ⁺细胞在白细胞介素2刺激后，检测其对K562靶细胞的细胞溶解活性。