通过逆转录酶促合成与线粒体信使核糖核酸互补的脱氧核糖核酸。
Enzymatic synthesis of DNA complementary to mitochondrial mRNA via reverse transcription.
作者信息
Frolova L, Arsenyan S, Avdonina T, Gaitskhoki V, Kisselev O, Neifach S, Kisselev L
出版信息
Nucleic Acids Res. 1978 Jan;5(1):285-95. doi: 10.1093/nar/5.1.285.
The poly(A)-containing mitochondrial mRNAs of rat liver were tested for their ability to serve as templates for the DNA synthesis by means of reverse transcription in the presence of the oligo(dT) primer and the RNA-directed DNA-polymerase from avian myeloblastosis virus. The mT-mRNA does not support the DNA synthesis in the standard conditions sufficient for effective reverse transcription of rabbit globin mRNA and of poly(A) in the presence of oligo(dT) primers. After a mild alkaline treatment of the mRNA and subsequent polyadenylation of the 3'-termini of the generated fragments with ATP:RNA adenyltransferase from E.coli the poly(A) (+) polyribonucleotides are able to serve as templates for reverse transcription in the presence of oligo(dT) and the reverse transcriptase. A conclusion is made that a "structural stop" exists in mitochondrial mRNA non-translable regions adjacent to the poly(A) terminal sequence. The "structural stop" is suggested to be caused by post-transcriptional modification of mRNA (methylation, etc.) or by a particularly stable secondary structure in this region of the mRNA molecules.
利用禽成髓细胞瘤病毒的寡聚（dT）引物和RNA指导的DNA聚合酶，通过逆转录检测大鼠肝脏中含聚腺苷酸（poly(A)）的线粒体mRNA作为DNA合成模板的能力。在足以有效逆转录兔珠蛋白mRNA和存在寡聚（dT）引物时的聚腺苷酸（poly(A)）的标准条件下，线粒体mRNA（mT-mRNA）不支持DNA合成。对mRNA进行温和的碱性处理，随后用来自大肠杆菌的ATP:RNA腺苷酸转移酶对产生的片段的3'末端进行聚腺苷酸化后，聚腺苷酸（poly(A)）(+)多聚核糖核苷酸能够在寡聚（dT）和逆转录酶存在的情况下作为逆转录的模板。得出的结论是，在线粒体mRNA与聚腺苷酸（poly(A)）末端序列相邻的不可翻译区域存在“结构终止”。“结构终止”被认为是由mRNA的转录后修饰（甲基化等）或mRNA分子该区域特别稳定的二级结构引起的。
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